Isolation and characterization of an estrogen-degrading Pseudomonas putida strain SJTE-1
3 BIOTECH
Authors: Wang, Pingping; Zheng, Daning; Liang, Rubing
Abstract
In this report, Pseudomonasputida SJTE1 isolated from an enrichment culture of sludge was confirmed to degrade natural estrogens (17 beta-estradiol, estrone, estriol), estrogenic chemicals (naphthalene and phenanthrene) and testosterone. The strain completely degraded 1 mg/L 17 beta-estradiol in 24h and transformed it into estrone; 90% and 75% of 50 mg/L and 100 mg/L 17 beta-estradiol were utilized in 7days, respectively. The transformation efficiency of this strain against natural estrogens was much higher than that against other estrogenic chemicals. Organic carbon sources, lipopolysaccharide and surfactants could enhance the degradation efficiency of strain SJTE-1 against 17-estradiol.The adsorption of 17-estradiol onto the biomass was the premise for transmembrane and cellular utilization of this chemical. This work has the potential to bioremediate the environmental estrogens.
Ab Initio Study of 17 alpha-Ethinylestradiol and Estrone Molecules Interacting with Single Wall Carbon Nanotube
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
Authors: Castro, Shuanne M.; Ribeiro, Herisson C.; Araujo, Adriana B.; Guerini, Silvete
Abstract
In this work, a systematic study of 17 alpha-ethinylestradiol and estrone molecules interacting with single wall carbon nanotube is presented by means of first principle calculations. The structural properties, binding energy, charge transfer, and electronic properties were calculated and analyzed to characterize the effect of interaction of 17 alpha-ethinylestradiol and estrone molecules on the nanotube surface. Results show that the molecular structure and electronic character of single wall carbon nanotube were not affected due to the presence of the molecules. It is observed via binding energy calculated that ethinylestradiol and estrone molecules interact weakly with the nanotube. The results are very interesting once that they suggest that nanotubes are sensitive to 17 alpha-ethinylestradiol and estrone molecules which can lead to confection of sensor to detect these molecules.