Sample
serum, EDTA-plasma, cell culture supernatant
Intended Use
The CD's Rat GRO/CINC-3 Assay Kit is a complete kit for the quantitative determination of Rat GRO/CINC-3 in serum, EDTA-plasma and supernatant of cell culture media.
Both recombinant and native forms of Rat GRO/CINC-3 can be detected with the kit.
Contents of Kit
1 Precoated plate: 96Well x 1
2 Labeled antibody Conc.: 0.4mL x 1
3 Standard: 0.5mL x 2
4 EIA buffer: 30mL x 1
5 Solution for Labeled antibody: 12mL x 1
6 Chromogen: 15mL x 1
7 Stop solution: x 1
8 Wash buffer Conc.: 50mL x 1
Detection Range
4.7 - 300 pg/mL
General Description
GRO (Growth Related Oncogene) belonging to IL-8 family is a polypeptide which has three isoforms (a, β and γ), and it inhibits proliferation of endothelial cells. GRO/CINC-1 (cytokine-induced neutrophil chemo attractant 1) was originally purified from media conditioned by IL-1β stimulated rat kidney epithelioid cells (NRK-52E). CINC-1 is a member of the alpha (CXC) subfamily of chemokines. Three additional rat CXC chemokines (CINC-2a, CINC-2β, CINC-3/MIP-2) have been identified. The protein sequence of CINC-1 is 63 - 67% identical to that of CINC-2a, CINC-2β and CINC-3/MIP-2. In addition, each of GROa, GROβ and GRO? is sharing 68%, 71% and 69% identity with CINC-1. This has been suggested that CINCs are the rat counterparts of human GROs. GROa/MGSA has a high homology with IL-8 in amino acid sequences.
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