Rat Cystathionine Gamma Lyase ELISA Kit

Individuals harbouring specific genetic variations might trend towards suffering sudden cardiac death. Cystathionine-gamma-lyase is one of the key enzymes of endogenous hydrogen sulfide production, and a key factor on the expression regulation of hydrogen sulfide in human heart. Compelling studies have suggested the cardioprotective effects of hydrogen sulfide, while it remains controversial whether cystathionine-gamma-lyase and hydrogen sulfide are beneficial to cardiovascular diseases. In this study, we performed a candidate-gene-based study to evaluate the association of the Indel polymorphism rs113044851 within the 3' untranslated region of Cystathionine-gamma-lyase gene and risk of sudden cardiac death in a Chinese Han population. Logistic regression analysis showed that the insertion allele of rs113044851 significantly decreased the risk of sudden cardiac death [odds ratio = 0.58; 95% confidence interval:0.38-0.88; P = 0.0076]. Further genotype-phenotype association analysis indicated that the insertion allele was significantly associated with lower expression of cystathionine-gamma-lyase in myocardium tissues. The subsequently in-silico predication revealed that compared with the deletion allele, the binding of the insertion allele with miR-1324 matched better. Finally, dual-luciferase activity assay validated the prediction that the gene transcriptional activity indicated by firefly luciferase activity with ins/ins genotype was lower than that with del/del genotype. In summary, our data suggested that rs113044851 might contribute to susceptibility of sudden cardiac death via regulating gene expression at post-transcriptional level. This indel has the potential to become a molecular diagnosis marker and genetic counseling of sudden cardiac death.

An effective catalytic transfer hydrogenation (CTH) process of bio-based levulinate esters into gamma-valerolactone (GVL) was explored over ternary Cu/ZnO/Al2O3 catalyst which was prepared by co-precipitation method and could be sustainably used. As a result, quantitative conversion of ethyl levulinate (EL) and 99.0% yield of GVL were obtained in the CTH process using i-PrOH as hydrogen donor. The Cu/ZnO/Al2O3 catalyst with high-surface-area could be reused at least four times without the loss of catalytic activity. Furthermore, the structure and properties of Cu/ZnO/Al2O3 catalyst was characterized through XRD, BET, SEM, TEM and H-2-TPR. Also, the influence of different support oxides and calcination temperatures was investigated. (c) 2018 Science Press and Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.