Detection Method
Indirect ELISA
Intended Use
The CSE1L Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CSE1L protein expression profile in cells. The kit can be used for measuring the relative amounts of CSE1L in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CSE1L.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-CSE1L Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 seals
Citations
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Tung, JN; Tsao, TY; et al. Distribution of Lysosome-associated Membrane Proteins-1 and-2, and Cathepsin D in Eosinophilic Granular Bodies: Possible Relationship to Cyst Development in Pilocytic Astrocytomas. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 38:1354-1364(2010).
Wellmann, A; Flemming, P; et al. High expression of the proliferation and apoptosis associated CSE1L/CAS gene in hepatitis and liver neoplasms: Correlation with tumor progression. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 7:489-494(2001).