CD47/SIRP-α (Biotiny) Inhibitor Screening Assay Kit

Intended Use

The CD47/SIRP-α Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47/SIRP-α interaction.

Contents of Kit

1. CD47, Fc fusion: 10 μg, -80°C;
2. SIRP-α (CD172a), His-tag, Biotin-labeled: 60 μg, -80°C;
3. Streptavidin-HRP: 15 μl, +4°C;
4. 3x CD47 Assay Buffer: 50 ml, -20°C;
5. Blocking Buffer: 50 ml, +4°C;
6. HRP chemiluminescent substrate A (transparent bottle):6 ml, +4°C;
7. HRP chemiluminescent substrate B (brown bottle): 6 ml, +4°C;
8. White 96-well microplate: 1, +4°C.

Storage

One year from date of receipt when stored as directed.

General Description

Signal-regulatory protein alpha (SIRP-α ), also known as CD172a, is a cell surface protein expressed mainly by myeloid cells such as macrophages. Its receptor, CD47, is ubiquitously expressed on the surface of all cells and has been found to be overexpressed in some cancers. CD47 binding to SIRP- α delivers a "Do Not Eat Me" signal to macrophages. In the context of a tumor, this signaling aids in tumor evasion of the immune system. Inhibitors of this interaction hold great promise for cancer immunotherapy.