Mouse Anti-Human Witronectin Receptors Hybridoma [C7I23.3]

Immunogen

Purified integrin associated protein (which is physically and functionally associated with vitronectin receptors)

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

P3X63Ag8.653

Clone

C7I23.3

Cell Line Description

Animals were immunized with purified integrin associated protein (which is physically and functionally associated with vitronectin receptors). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with vitronectin receptors on human polymorphonuclear leukocytes (PMN, neutrophils) and blocks the receptor binding to the tripeptide Arg-Gly-Asp (RGD). The antibody also inhibits RGD stimulated phagocytosis. It precipitates a heterodimer of apparent molecular weights 140000 and 105000 daltons. The antibody does not react with gpIIb/III or the LFA-1/Mac-1 family of cellular adhesion receptors.

Morphology

Lymphoblast

Growth Properties

Suspension

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.

Synonyms

CD47; CD47 molecule; CD47 antigen (Rh related antigen, integrin associated signal transducer), MER6; leukocyte surface antigen CD47; antigen identified by monoclonal 1D8; antigenic surface determinant protein OA3; CD47 glycoprotein; IAP; integrin associated protein; OA3; Rh related antigen; Rh-related antigen; integrin-associated protein; integrin-associated signal transducer; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); MER6;

Entrez Gene ID

UniProt ID