Anti-CD47 monoclonal antibody, clone C7I23.3

Antibody Isotype

IgG1

Clone

C7I23.3

Species Reactivity

Human

Immunogen

Intact CD47 purified from human placenta.

Conjugate

Application Notes

Flow Cytometry (0.5-1 ug/million cells);
Immunofluorescence (1-2 μg/mL);
Immunohistochemistry (Frozen sections) (0.5-1 μg/mL for 30 min at RT);
Immunoprecipitation (1-2 ug/500 ug protein);
WB (0.25-0.5 μg/mL) & The optimal working dilution should be deter

Alternative Names

CD47; CD47 molecule; CD47 antigen (Rh related antigen, integrin associated signal transducer) , MER6; leukocyte surface antigen CD47; antigen identified by monoclonal 1D8; antigenic surface determinant protein OA3; CD47 glycoprotein; IAP; integrin associ

Entrez Gene ID

UniProt ID

Antigen Description

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Pathway

Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Hemostasis, organism-specific biosystem; Integrins in angiogenesis, organism-specific biosystem; Signal regulatory protein (SIRP) family interactions, organism-specific biosystem;