Porcine Terminal complement complex C5b-9 ELISA Kit

Name: Porcine Terminal complement complex C5b-9 ELISA Kit
SKU: DEIA-BJ2847
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, tissue homogenates and other biological fluids.

Species Reactivity

Porcine

Detection Method

sELISA

Intended Use

For quantitative detection of TCC C5b-9 in serum, plasma, tissue homogenates and other biological fluids.

Contents of Kit

1. ELISA Microplate (Dismountable): 8×12, 2-8°C/-20°C
2. Lyophilized Standard: 2 vials, 2-8°C/-20°C
3. Sample Dilution Buffer: 20 ml, 2-8°C
4. Biotin-labeled Antibody(Concentrated): 120 μl, 2-8°C (Avoid Direct Light)
5. Antibody Dilution Buffer: 10 ml, 2-8°C
6. HRP-Streptavidin Conjugate (SABC): 120 μl, 2-8°C (Avoid Direct Light)
7. SABC Dilution Buffer: 10 ml, 2-8°C
8. TMB Substrate: 10 ml, 2-8°C (Avoid Direct Light)
9. Stop Solution: 10 ml, 2-8°C
10. Wash Buffer (25X): 30 ml, 2-8°C
11. Plate Sealer: 5 pieces
12. Product Description: 1 copy

Storage

2-8°C/-20°C

Precision

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Detection Range

7.813-500 ng/ml

Detection Limit

4.688 ng/ml

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Comparative anatomy of the brachial plexus and shoulder nerves of theSapajus

JOURNAL OF MEDICAL PRIMATOLOGY

Authors: Figueredo-da-Silva, Sylla; Vasconcelos-da-Silva, Ediana; Abreu, Taina; Aversi-Ferreira, Tales Alexandre

Abstract

Background The capuchin is a neotropical primate that presents easy reproduction in captivity and is used in scientific research. The objective of this work was to describe the structure and the branching pattern of the brachial plexus of the capuchin and to compare the results with data from the literature for humans, chimpanzees, baboons andCallithrix. Methods Twelve specimens were used: eight males and four females. No animals were killed for the purpose of this study. Results The brachial plexus ofSapajussp was constituted mainly from the ventral roots of the last four cervical spinal nerves, from C5 to C8, mainly, and the first thoracic nerve (T1). Conclusions The pattern of formation of the brachial plexus of the capuchin monkey was more similar to that ofCallithrixand baboons, perhaps because they are the only primates in this study to be essentially quadrupedal.

Interference of the Zika Virus E-Protein With the Membrane Attack Complex of the Complement System

FRONTIERS IN IMMUNOLOGY

Authors: Malekshahi, Zahra; Schiela, Britta; Bernklau, Sarah; Banki, Zoltan; Wuerzner, Reinhard; Stoiber, Heribert

Abstract

The complement system has developed different strategies to clear infections by several effector mechanisms, such as opsonization, which supports phagocytosis, attracting immune cells by C3 and C5 cleavage products, or direct killing of pathogens by the formation of the membrane attack complex (MAC). As the Zika virus (ZIKV) activates the classical complement pathway and thus has to avoid clearance by the complement system, we analyzed putative viral escape mechanisms, which limit virolysis. We identified binding of the recombinant viral envelope E protein to components of the terminal pathway complement (C5b6, C7, C8, and C9) by ELISA. Western blot analyses revealed that ZIKV E protein interfered with the polymerization of C9, induced on cellular surfaces, either by purified terminal complement proteins or by normal human serum (NHS) as a source of the complement. Further, the hemolytic activity of NHS was significantly reduced in the presence of the recombinant E protein or entire viral particles. This data indicates that ZIKV reduces MAC formation and complement-mediated lysis by binding terminal complement proteins to the viral E protein.