Anti-C3 polyclonal antibody

Managed aquifer recharge (MAR), an intentional storage of excess water to an aquifer, is becoming a promising water resource management tool to cope with the worldwide water shortage. Bioclogging is a commonly encountered operational issue that lowers hydraulic conductivity and overall performance in MAR. The current study investigates the impact of carbon and nitrogen in recharge water on bioclogging in MAR. For this investigation, continuous-flow columns packed with sand grains were operated with influents having 0 (C1), 5 (C2), and 100 mg/L (C3) of glucose with or without introduction of nitrate. Hydraulic conductivity was analyzed to evaluate bioclogging in the systems. In C1 and C2, hydraulic conductivity was not significantly changed overall. However, hydraulic conductivity in C3 was decreased by 28.5% after three weeks of operation, which appears to be attributed to generation of fermentation bacteria. Introduction of nitrogen to C3 led to a further decrease in hydraulic conductivity by 25.7% compared to before it was added, most likely due to stimulation of denitrifying bacteria. These findings indicate that high carbon contents and introduction of additional nitrogen in recharge water cause serious bioclogging in MAR, suggesting the necessity for controlling quality of recharge water.

Intermittent rain events drive dynamic pulses of carbon and water exchange in many arid and semiarid ecosystems. Although soil moisture is known to control these pulses, the effect of atmospheric dryness on pulses is not well documented. Here we hypothesized that vapor pressure deficit (VPD) modulates net ecosystem production (NEP) and ecosystem-scale water use efficiency (WUE) during pulse events due to its effects on canopy stomatal conductance and evapotranspiration. We quantified relationships between VPD and carbon and water exchange during growing season rain events and tested their generality across four semiarid flux sites with varied vegetation in the southwest United States. Across grassland, shrubland, and savanna sites, we found that high VPD during pulses suppressed ecosystem photosynthesis and surface conductance to a greater degree than respiration or evapotranspiration, particularly when soil moisture was high. Thus, periods of high VPD were associated with a 13-64% reduction in NEP and an 11-25% decrease in WUE, relative to moderate VPD conditions. Sites dominated by shrubs with the C3 photosynthetic pathway were more sensitive to VPD than sites dominated by C4 grasses. We found that a 1 kPa increase in VPD reduced the average NEP of pulse events by 13-56%, which illustrates the potential for projected increases in atmospheric demand to reduce the net productivity of semiarid ecosystems.