Human C3d (DAGF-017)

Human C3d from normal human serum Datasheet

Online Inquiry Add to basket

Molecular Weight
33.8 kDa
Alternative Names
>90% by SDS-PAGE
Batch dependent - please inquire should you have specific requirements.
100 µg
10 mM Sodium phosphate, 145 mM NaCl, pH 7.3
Store at -70°C or below. Avoid freeze/thaw.
Antigen Description
C3d is derived from human C3 after a series of proteolytic cleavages. Trypsin-like enzymes (such as plasmin, thrombin, elastase, etc.) cleave iC3b separating C3c from C3dg (38,900 Da) and C3dg is further digested releasing C3g (5,100 Da) to leave C3d. iC3b (inactivated C3b) is derived from C3b. C3b itself is produced by all three pathways of complement (Law, S.K.A. and Reid, K.B.M. (1995)) when native C3 is cleaved releasing C3a. C3d is prepared at CompTech from purified human C3. The C3 is converted to C3b by treatment with the natural human C3 convertase and this C3b is converted to iC3b by treatment with complement factors H and I. Finally, this iC3b is cleaved with trypsin to yield C3d. The C3d made by CompTech is made from soluble C3b. If the C3b precursor was attached to a surface, such as occurs during complement activation, the iC3b, C3dg and C3d fragments would remain covalently attached to that surface. C3c is released into the fluid phase. The C3d sold by CompTech is not capable of attaching to a surface. This can only occur during complement activation on a surface. Surface-bound C3d is linked to the target through an ester or an amide covalent bond. Ester bonds are unstable resulting in the gradual release of C3d from the particle.


Have you cited DAGF-017 in a publication? Let us know and earn a reward for your research.

Related Products

Human C3c (DAGF-016)
Human IC3b (DAG4658)
Human C3b (DAG4657)

Customer Reviews

Write a review, share your experiences with others and get rewarded !

Online Inquiry

Phone *
E-mail Address *
Service & Products Interested *
Project Description
Verification Code * Please input "diagnostics" as verification code.

Online Inquiry

Order Info: Human C3d

Online Inquiry
  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!
  15% off your first purchase

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket