Human Complement C3 ELISA Kit

Name: Human Complement C3 ELISA Kit
SKU: DEIA9417
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

plasma, serum, urine, milk, saliva, cell culture samples

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The Human Complement C3 ELISA Kit is intended for the quantitative determination of total complement C3 antigen in human plasma, serum, urine, milk, saliva and cell culture samples.

Contents of Kit

1. 96-well microtiter strip plate: 8 x 12 removable well strips containing anti-human complement 3 antibody on the surface. Strips are blocked and dried.
2. 10X Wash Buffer: 1 bottle of 50 mL; bring to 1 x using DI water
3. Human complement C3 Standard: 1 vial of lyophilized Standard
4. Anti-human C3 primary antibody: 1 vial of lyophilized antibody
5. Peroxidase conjugated streptavidin: 1 vial of concentrated secondary reagent
6. TMB substrate solution: 1 bottle of 10 mL solution

Storage

All kit components must be stored at 4°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 4°C. Kit should be used no later than the expiration date.

Precision

Detection Range

The assay measures total human complement C3 in the 0.02-10 ng/mL range.

Sensitivity

0.0117 ng/mL

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

The Arabidopsis R2R3 MYB Transcription Factor MYB15 Is a Key Regulator of Lignin Biosynthesis in Effector-Triggered Immunity

FRONTIERS IN PLANT SCIENCE

Authors: Kim, Seu Ha; Lam, Pui Ying; Lee, Myoung-Hoon; Jeon, Hwi Seong; Tobimatsu, Yuki; Park, Ohkmae K.

Abstract

Lignin, a major component of the secondary cell wall, is important for plant growth and development. Moreover, lignin plays a pivotal role in plant innate immunity. Lignin is readily deposited upon pathogen infection and functions as a physical barrier that limits the spread of pathogens. In this study, we show that an Arabidopsis MYB transcription factor MYB15 is required for the activation of lignin biosynthesis genes such asPAL,C4H,4CL,HCT,C3 ' H,COMT, andCAD, and consequently lignin formation during effector-triggered immune responses. Upon challenge with the avirulent bacterial pathogenPstDC3000 (AvrRpm1), lignin deposition and disease resistance were reduced inmyb15mutant plants. Furthermore, whereas invading pathogens, together with hypersensitive cell death, were restricted to the infection site in wild-type leaves, they spread beyond the infected area inmyb15mutants. The exogenous supply of the lignin monomer coniferyl alcohol restored lignin production and rescued immune defects inmyb15plants. These results demonstrate that regulation at the transcriptional level is key to pathogen-induced lignification and that MYB15 plays a central role in this process.

Association between the presence of distolingual root in mandibular first molars and the presence of C-shaped mandibular second molars: a CBCT study in a Taiwanese population

QUINTESSENCE INTERNATIONAL

Authors: Wu, Yu-Chiao; Su, Wen-Song; Mau, Lian-Ping; Cheng, Wan-Chien; Weng, Pei-Wei; Tsai, Yi-Wen Cathy; Su, Chi-Chun; Chiang, Ho-Sheng; Lin, Jarshen; Shieh, Yi-Shing; Huang, Ren-Yeong

Abstract

Objectives:The purpose of this study was to assess the prevalence of C-shaped canals in permanent mandibular second molars (SMs) and to determine whether its appearance was associated with the presence of distolingual root (DLR) in permanent mandibular first molars (FMs). Method and materials: Three hundred and eighty patients were qualified for evaluation of their FMs and SMs using cone beam computed tomography.The prevalence, distribution pattern, external root morphology, and the internal root canal anatomy of the examined molars were recorded and analyzed. Furthermore, the association between the root canal configurations of SMs and the appearance of DLR in FMs was also assessed. Results: The prevalence of SMs with C-shaped root canals was 44.7%. The most common root canal configuration type of the one-rooted SMs with C-shaped anatomy was C3 (45.6%), followed by C2 and C1.The frequency of C-shaped canals in SMs was 45.4%in Non-DLR group, 52.6% in unilateral DLR group, and 33.9% in bilateral DLR group, respectively. Moreover, the prevalence of C-shaped root canals in SMs with the presence of bilateral DLRs in FMs was significantly lowered. Conclusion:The association between the presence of DLR in FMs and C-shaped canal configurations in neighboring SMs was surveyed, and the prevalence of C-shaped root canals in SMs with the presence of bilateral DLRs in FMs was found to be significantly lowered.