Intended Use
The Bcl-6 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Bcl-6 protein expression profile in cells. The kit can be used for measuring the relative amounts of Bcl-6 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Bcl-6.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate, 1 Plate.
2. 10× TBS, 25 ml (10×)
3. Quenching Buffer, 25 ml (1×)
4. Blocking Buffer, 50 ml, (1×)
5. 15× Wash Buffer, 50 ml (15×)
6. 100× Anti- Target(Bcl-6) Antibody, 60 μl (100×)
7. 100× Anti-GAPDH Antibody, 60 μl (100×)
8. HRP-Conjugated, Anti-Rabbit IgG Secondary Antibody, 6 ml (1×)
9. HRP-Conjugated, Anti-Mouse Secondary IgG Antibody, 6 ml (1×)
10. Primary Antibody Diluent, 12 ml (1×)
11. Ready to Use Substrate, 12 ml (1×)
12. Stop Solution, 12 ml (1×)
13. Crystal Violet Solution, 6 ml (1×)
14. SDS Solution, 25 ml (1×)
15. Adhesive Plate Seals, 2 Seals
COA
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