Bacitracin ELISA kit

Sample

Shrimp, pork, beef, chicken, egg, and milk samples

Species Reactivity

NA

Detection Method

cELISA

Intended Use

The Bacitracin ELISA Test Kit is a competitive immunoassay to quantitatively detect the presence of BAC in shrimp, pork, beef, chicken, egg, and milk samples.

Contents of Kit

1. Pre-coated microtitre plate, 12 x 8 wells
2. BAC antibody solution, 1 x 7 ml
3. Enzyme conjugate, 1 x 7 ml
4. Wash buffer (20x concentrate), 1 x 30 ml
5. Assay diluent (5x concentrate), 1 x 30 ml
6. Tissue Sample Preparation Reagent, 2 x 50 ml
7. BAC standards, (0, 3, 9, 27,81, 243 ppb), 0.5 ml each
8. High concentrate of BAC standard, (1000 ppb)* 1 x 1 ml
9. TMB solution (ready to use) 1 x 12 ml
10. Stop solution (ready to use) 1 x 12 ml
11. Microplate sealer 1 piece
12. Package insert 1 copy
* This component is optional and only for the user to check the recovery rate of BAC.

Storage

• The kit should be stored at 2–8°C. Do not freeze.
• Unused test wells should be sealed and stored at 2–8°C.
• This kit is valid until the expiration date printed on the label.

Detection Limit

Shrimp sample: 90 ppb
Pork sample: 90 ppb
Beef sample: 90 ppb
Chicken sample: 90 ppb
Egg sample: 90 ppb
Milk samples: 30 ppb

General Description

Bacitracin (BAC) is a polypeptide antibiotic. In addition to veterinary applications, BAC can be added to feed and used as an animal growth promoter. Whenever these drugs are administered to food producing animals, residues in milk, eggs or edible tissues are likely. These residues can cause allergic reactions but also promote antibiotic resistance of bacteria which may pose a health risk for the population.