Rabbit Anti-Human BRCA2 (internal) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

A synthesized peptide derived from human BRCA2, corresponding to a region within the internal amino acids.

Conjugate

Application Notes

WB 1:500-1:2000
IF/ICC 1:100-1:500

General Notes

This product (Catalog No. CABT-BL458) is a rabbit-derived polyclonal antibody raised against human BRCA2. This antibody exhibits cross-reactivity with BRCA2 from mouse and rat. It has been affinity purified by Protein A. BRCA2, also known as breast cancer 2 early onset, is a human tumor suppressor gene located on the long arm of chromosome 13 at position 13q13.1 that encodes a large multifunctional protein critical for maintaining genomic integrity through its role in homologous recombination (HR)-mediated repair of DNA double-strand breaks, as well as in replication fork protection and cell cycle checkpoint regulation. The protein interacts with RAD51 recombinase to facilitate strand invasion during HR, preventing chromosomal instability that can lead to tumorigenesis. Germline pathogenic variants in BRCA2, first identified in 1995 through positional cloning in high-risk breast cancer families, are associated with hereditary breast and ovarian cancer syndrome (HBOC), conferring empirically observed cumulative lifetime risks of approximately 45-69% for female breast cancer, 11-17% for ovarian cancer, and elevated risks for male breast (up to 8%), prostate (15-20% by age 65), and pancreatic cancers. These mutations disrupt DNA repair fidelity, leading to characteristic genomic scarring such as loss of heterozygosity and high tumor mutational burden, which underpin synthetic lethality with PARP inhibitors in therapeutic contexts. Despite comprehensive sequencing efforts revealing over 3,000 distinct variants, challenges persist in variant classification due to incomplete penetrance and modifier effects, underscoring the need for prospective cohort data over retrospective estimates in risk modeling.
CABT-BL458 is suitable for use in WB 1 (500-1:2000), IF/ICC 1 (100-1:500) applications.
CABT-BL458 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Entrez Gene ID

UniProt ID