Human/Mouse/Rat Pro-BDNF ELISA Kit

Sample

serum

Species Reactivity

Human

Intended Use

For the quantitative determination of human pro-BDNF(19-24) concentrations in serum.

Storage

##Unopened Kit##: Store at 2 - 8°C for up to 6 months. For longer storage, unopened Standard, Positive Control and Detection Antibody Concentrate should be stored at -20°C or -70°C. Do not use kit past expiration date.
##Opened / Reconstituted Reagents##: Reconstituted Standard (stock) and Detection Antibody concentrated solution SHOULD BE STORED at -20°C or -70°C for up to one month. Streptavidin-HRP Conjugate 100-fold concentrated solution (protect from light) and other components may be stored at 2 - 8°C for up to 6 months. Do not freeze TMB substrate solution or Streptavidin-HRP.
##Microplate Wells##: Return unused wells to the plastic pouch (P01) with desiccant pack. Microplate may be stored at 2 - 8°C for up to 6 months.

Precision

Intra-assay Precision: 4-6%;
Inter-assay Precision: 8-10%

Detection Range

1.56 - 100 ng/ml

Sensitivity

0.5 ng/ml

General Description

This Human Pro-BDNF ELISA Kit contains the necessary components required for the quantitative measurement of recombinant human Pro BDNF (19-247) (human cells derived) and/or natural human Pro-BDNF (19-247) from serum samples in a sandwich ELISA format.
This immunoassay contains human Pro-BDNF (19-247) recombinant and antibodies raised against this protein. Results from this immunoassay have shown to accurately quantify bioactive recombinant and natural Pro-BDNF (19-247) in the samples. The mature form BDNF or Pro BDNF (19-128) in the samples cannot be detected by this elisa kit.