Human pro-BDNF(pro Brain Derived Neurotrophic Factor) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of pro-BDNF concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

1. ELISA Microplate(Dismountable), 8×12, Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 6 month at -20°C.
2. Lyophilized Standard, 2vial, Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 6 month at -20°C.
3. Biotin-labeled Antibody(Concentrated, 100×), 120ul, 2-8°C (Avoid Direct Light)
4. HRP-Streptavidin Conjugate(SABC, 100×), 120ul, 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml, 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer, 20ml, 2-8°C
7. Antibody Dilution Buffer, 10ml, 2-8°C
8. SABC Dilution Buffer, 10ml, 2-8°C
9. Stop Solution, 10ml, 2-8°C
10. Wash Buffer(25×), 30ml, 2-8°C
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

15.625-1000pg/ml

Sensitivity

9.375pg/ml

General Description

Neurotrophins are composed of at least four family members, including NGF, BDNF, NT-3, and NT-4, and all are known to influence growth, development, differentiation, and survival of neurons . Proneurotrophins bind to p75NTR, but not to the family of Trk receptor tyrosine kinases (Trk), and following maturation, BDNF binds and activates TrkB. Trk receptors, in turn, activate three major signaling pathways: (a) Ras-MAPK signaling, which promotes neuronal differentiation and neurite outgrowth, (b) PI3K/Akt signaling, which promotes survival and growth of neurons, and (c) PLC-γ1-PKC signaling, which promotes synaptic plasticity. BDNF is a major regulator of transmission and plasticity at adult synapses.

Standard Curve

This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C-25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.