6-Panel Drug Test (Strip) ( BAR, BZD, COC, MAD, MOR, THC ) (DTS319)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Sample
urine
Intended Use
All of DOA Panel Drug Test is an immunochromatography based one step in vitro test. It is designed for qualitative determination of drug substances in human urine specimens. This assay may be used in the point of care setting. Below is a list of cut-off concentrations for each drug using our test.
Storage
The test device should be stored at 2 to 30°C and will be effective until the expiration date stated on the package. The product is humidity-sensitive and should be used immediately after being open. Any improperly sealed product should be discarded.
Sensitivity
The cut-off concentrations (sensitivity level) of DOA Panel Drug Test are determined to be: AMP 1000 ng/ml, BAR, 300 ng/ml, BZO 300 ng/ml, BUP 10 ng/ml, COC 300 ng/ml, EDDP 100 ng/ml, KET 1000 ng/ml, MTD 300 ng/ml, MET 1000 ng/ml, MDMA 500 ng/ml, OPI 300

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References


HepatoprotectiveAngelica sinensissilver nanoformulation against multidrug resistant bacteria and the integration of a multicomponent logic gate system

NANOSCALE

Authors: Afthab, Jouharsha; Khatoon, Nafeesa; Zhou, Lulu; Yao, Tianming; Shi, Shuo

The rampant usage of antibiotics has led to the emergence of toxicity, especially hepatotoxicity and the emergence of microbial drug resistance. Hence, a series of novel hepatoprotective, biocompatible, antibacterial silver nanoformulations (AS-AgNPs) were developed by using the important Chinese medicinal plantAngelica sinensis. The different size of AS-AgNPs were characterized by UV-Visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The size-dependent antibacterial properties of AS-AgNPs were investigated against Gram-positive, Gram-negative and multi-drug resistant bacteria. The minimum inhibitory concentration (MIC) of AS-AgNPs with different size against six bacteria was found to be in the range of 5-100 mu g mL(-1)with no resistance till 12 cycles. TEM and SEM results of bacteria after the treatment suggested that AS-AgNPs disrupted the cell membrane by creating pores. The cytocompatibility and cytoprotective effect of AS-AgNPs were evaluated against HepG2 cell lines, which showed that 85% of cells were viable up to 100 mu g mL(-1)of the concentration with almost no change in AST and ALT levels. Further, a logic combinatorial library, including basic logic gates (AND, OR, NOR, INHIBIT, IMPLICATION, and YES), three input logic gates (OR, and NOR) and combinatorial gates (INH-OR, INH-YES, INH-INH, AND-NOR, and NOT-AND-NOR) were designed by integrating multi-components based on the interaction between AS-AgNP1 and bacteria, where DiSC(3)(5) was used as the signal reporter. This system clearly demonstrates the ability of simple logic circuits to perform sophisticated analysis for the detection of multiple bacteria.

Identification of Non-tuberculous Mycobacteria by Partial Gene Sequencing and Matrix Assisted Laser Desorption Ionisation Time of Flight at a Tertiary care Hospital Telangana, India

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH

Authors: Angaali, Neeliam; Kadasu, Rajashekar; Patil, Madhusudhan Apparao; Teja, Vijay Dharma

Introduction: Non-tuberculous Mycobacteria (NTM) pulmonary disease is often unrecognised or misdiagnosed as Mycobacterium tuberculosis (MTB), Multi-Drug Resistant Tuberculosis (MDRTB), because of similar clinical presentation in counties with high burden of Tuberculosis (TB) including India. In India due to lack of awareness among clinicians and lack of laboratory facilities to diagnose these infections its prevalence is largely unknown. Aim: To evaluate the efficacy of identification of NTM species by Matrix Assisted Laser Desorption Ionisation Time of Flight (MALDI-TOF) and Heat Shock Protein (hSP65) gene sequencing and to determine their Antimicrobial Susceptibility Testing (AST). Materials and Methods: All the clinical specimens from pulmonary and extra-pulmonary TB suspects at Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India, over a period of one year i.e., from June 2017 to May 2018 were included in the study. The specimens were subjected to microscopy, culture and GeneXpert. The NTMs isolated in the culture were further characterised genotypically by MALDI-TOF and hsp65 gene sequencing. The identified NTM isolates were subjected to AST. All the methods were followed as per the standard protocols. Data was analysed using SPSS 25. Results: A total of 1085 samples were processed out of which Mycobacteria was detected in 201 cases (18.5%). Among the culture positives, MTB complex was detected in 146 cases (135%) and NTM in 55 (5.06%). Mycobacterium abscessus was the predominant isolate. The most common co-morbidities were bronchiectasis and Chronic Obstructive Pulmonary Disease (COPD). Linezolid, clarithromycin, moxifloxacin and amikacin showed high sensitivity. Conclusion: Molecular assay helps in rapid identification which can lead to targeted therapy and can thus combat antimicrobial resistance. The MALDI-TOF and hsp65 gene sequencing also offers quick results at a low cost and is easy to perform hence it can be considered as an alternate diagnostic tool for identification.

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