Tag/Conjugate
Unconjugated
Endotoxin
The endotoxin content, determined using a kinetic chromagenic LAL assay, is approximately 20 EU/mg.
Alternative Names
Bordetella pertussis toxin
Purity
When examined on 16% Tricine gels in the prisence of a reducing agent, this preparation migrates as five distinct bands. Densitometric analysis estimates the purity as >95%.
Format
Lyophilized power, sealed under vacuum
Storage
Store at 2-8°C prior to and following reconstitution. DO NOT FREEZE.
Reconstitution
Reconstitute with water or buffer of choice. Mix gently after reconstitution to ensure a uniform suspension. Do not sterile filter, as this will result in loss of material. Handle the product gently; do not vortex.
Antigen Description
Pertussis toxin (PT), a virulence factor produced by Bordetella pertussis, is a multi-subunit toxin which binds to most cultured mammalian cells and targets specific G protein, inhibiting the ability of the G protein to function in signaling pathways. Depending on the function of the G protein, the effects of PT can vary. This ability to inhibit pathways utilizing the Gi family of protein-coupled receptors is the basis of the use of PT as a tool in cell biology. PT plays a role in infection by suppressing and modulating various host immune responses to Bordetella pertussis. PT has been used to stimulate experimental autoimmune diseases in rodent models, such as experimental auto immune encephalitis (EAE).
Citations
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COA
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