Mouse anti-Bovine ssDNA monoclonal antibody for FC, IHC
most DNA molecules are actually two polymer strands, bound together in a helical fashion by noncovalent bonds; this double stranded structure (dsDNA) is maintained largely by the intrastrand base stacking interactions, which are strongest for G,C stacks. The two strands can come apart – a process known as melting – to form two ssDNA molecules. Melting occurs when conditions favor ssDNA; such conditions are high temperature, low salt and high pH (low pH also melts DNA, but since DNA is unstable due to acid depurination, low pH is rarely used).
PARKER, W; BRUNO, D; et al. CHARACTERIZATION AND AFFINITY ISOLATION OF XENOREACTIVE HUMAN NATURAL ANTIBODIES. JOURNAL OF IMMUNOLOGY 153:3791-3803(1994).
Makowski, GS; Ramsby, ML; et al. Selective detection of autoimmune antibodies to single- and double-stranded DNA by enzyme immunoassay. ANNALS OF CLINICAL AND LABORATORY SCIENCE 33:142-148(2003).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.