Mouse Anti Chicken Creatine Kinase Hybridoma [DL-KBD]

Although considerable effort has been devoted to building commonsense knowledge bases (CKB), it is still not available for many low-resource languages such as Uyghur because of expensive construction cost. Focusing on this issue, we proposed a cross-lingual knowledge-projection method to construct an Uyghur CKB by projecting ConceptNet's Chinese facts into Uyghur. We used a Chinese-Uyghur bilingual dictionary to get high-quality entity translation in facts and employed a back-translation method to eliminate the entity-translation ambiguity. Moreover, to tackle the inner relation ambiguity in translated facts, we made a hand-crafted rule to convert the structured facts into natural-language phrases and built the Chinese-Uyghur lingual phrases based on the similarity of phrases that corresponded to the bilingual semantic similarity scoring model. Experimental results show that the accuracy of our semantic similarity scoring model reached 94.75% for our task, and they successfully project 55,872 Chinese facts into Uyghur as well as obtain 67,375 Uyghur facts within a very short period.

Onconeural immunity, a cancer-stimulated immune reaction that cross-reacts with neural tissues, is considered to be the principal pathological mechanism for paraneoplastic neurological syndromes (PNS). A common PNS is paraneoplastic cerebellar degeneration (PCD). We had encountered a PCD patient with urothelial carcinomas (UC) of the urinary bladder who was negative for the well-characterized PNS-related onconeural antibodies. In the present study, we aimed to identify a new PCD-related onconeural antibody, capable of recognizing both cerebellar neurons and cancer tissues from the patient, and applied a proteomic approach using mass spectrometry. We identified anti-creatine kinase, brain-type (CKB) antibody as a new autoantibody in the serum and cerebrospinal fluid from the patient. Immunohistochemistry indicated that anti-CKB antibody reacted with both cerebellar neurons and UC of the urinary bladder tissues. However, anti-CKB antibody was not detected in sera from over 30 donors, including bladder cancer patients without PCD, indicating that anti-CKB antibody is required for onset of PCD. We also detected anti-CKB antibody in sera from three other PCD patients. Our study demonstrated that anti-CKB antibody may be added to the list of PCD-related autoantibodies and may be useful for diagnosis of PCD. (C) 2013 Elsevier B.V. All rights reserved.