Human Creatine Kinase BB Isoenzyme (DAG279)

Human Creatine Kinase BB Isoenzyme, recombinant protein from P. pastoris Datasheet

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Product Overview
CreatineKinase BB (CK-BB) Isoenzyme Recombinant. Full length Creatine Kinase BBisoenzyme with amino acid sequence identical to the native enzyme. CKBB is a47 kDa dimeric protein comprised of 2 identical B subunits. Purified in theenzymatically active form
Purified, Liquid
Lotspecific. Activity: Lot specific IU/mg. One unit will transfer one micromoleof phosphate from creatine phosphate to ADP per minute at 37oC. Measured at340nm as one equimolar amount of NADH produced by coupled reaction.
10mMBis-Tris-HCl, 0.5mM DTT, 0.5mM EDTA, 50% glycerol, pH 6.0 + 0.2
2-8°C short term, -20°C long term
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized
Antigen Description
CreatineKinase MB consists of a dimer of nonidentical chains. With MM being the majorform in skeletal muscle and myocardium, MB existing in myocardium, and BBexisting in many tissues, especially brain. Creatine Kinase MB reversiblycatalyses the transfer of phosphate between ATP and various phosphogens. Thecreatine kinase isoenzymes play a central role in energy transduction intissues with large fluctuating energy demands such as skeletal muscle, heart,brain and spermatozoa.
CKB; creatine kinase, brain; CKBB; creatine kinase B-type; creatine kinase-B; creatine kinase B chain; B-CK;


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