Rat Anti-Mouse Zp3 Heterohybridoma [JF-21]

Immunogen

Particulate murine zonae pellucidae

Fusion Species

Rat X Mouse Heterohybridoma

Immunological Donor

Rat spleen

Myeloma

Sp2/0

Clone

JF-21

Cell Line Description

Animals were immunized with particulate murine zonae pellucidae. Spleen cells were fused with Sp2/0 mouse myeloma cells. During development and maturation, mammalian oocytes are surrounded by the zona pellucida, which in the mouse is comprised of three sulfated glycoproteins, ZP1, ZP2 and ZP3. The zona pellucida mediates sperm binding at fertilization, provides a post-fertilization block to polyspermy, and facilitates passage of pre-implantation embryos down the oviduct. The antibody specifically binds to mouse ZP3 glycoprotein (amino acids 336-342). The antibody cross-reacts with rat zonae but not with non-rodent species. Following passive immunization, this antibody completely blocks both in vivo and in vitro fertilization of subsequently ovulated mouse eggs.

Morphology

Lymphoblast

Growth Properties

Suspension

Storage

Liquid nitrogen vapor phase.

Synonyms

ZP3; zona pellucida glycoprotein 3; zona pellucida sperm-binding protein 3; sperm receptor; zona pellucida protein C; zona pellucida glycoprotein ZP3; Zp-3;

Entrez Gene ID

22788