Anti-Ubiquitin monoclonal antibody, clone 94567

Name: Anti-Ubiquitin monoclonal antibody, clone 94567
SKU: DCABY-4229
Rating: 5 (1 reviews)

Mouse Anti-Human Ubiquitin monoclonal antibody for WB, ELISA(Cap)

Write a review

COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG2b

Clone

94567

Species Reactivity

Human

Immunogen

E. coli-derived recombinant human Ubiquitin+1. Met1-Gly75, YADLREDPDRQDHHPGSGAQ (C-terminal sequence generated by a frameshift) Accession Number CAA44911

Conjugate

Unconjugated

Applications

Application Notes

Western Blot: 1 μg/mL; ELISA Capture: 2-8 μg/mL; ELISA Detection: 0.5-2.0 μg/mL
We recommend the following for sandwich ELISA (Capture - Detection):
DCABY-4229 - DCABY-4303

Target

Alternative Names

UBB; ubiquitin B; polyubiquitin-B; polyubiquitin B;

Entrez Gene ID

7314

UniProt ID

P0CG47

Product Background

Antigen Description

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin. In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting.

Pathway

APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Cyclin B; APC/C:Cdc20 mediated degradation of Securin; APC/C:Cdc20 mediated degradation of mitotic proteins; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cd

Citations

Have you cited DCABY-4229 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Set membership identification using SLLE and NMC

2010 8TH WORLD CONGRESS ON INTELLIGENT CONTROL AND AUTOMATION (WCICA)

Authors: Chai, Wei; Sun, Xianfang; Qiao, JunFei

Abstract

A set membership identification method by pattern classification is proposed for nonlinear-in-parameter regression models with unknown but bounded(UBB) noises. Suppose that the points in the parameter space can be divided into two classes according to whether they are in the feasible solution set or not, the problem of set membership identification is to construct a pattern classifier to decide which class a point belongs to. The method has three steps. Firstly, the training data are selected uniformly in the parameter space and are decided by equation error whether they are in the feasible solution set. Secondly, supervised locally linear embedding(SLLE) is used to map the training data into low-dimensional space. Thirdly, nearest mean classifier(NMC) is trained on the mapped training data. This method not only can describe the feasible solution set approximately in the high-dimensional parameter space, but also can characterize it in the low-dimensional feature space. Simulation results show the effectiveness of the proposed method.

Identification of reference genes for normalization of gene expression in thoroughbred and Jeju native horse(Jeju pony) tissues

GENES & GENOMICS

Authors: Ahn, Kung; Bae, Jin-Han; Nam, Kyu-Hwi; Lee, Chong-Eon; Park, Kyung-Do; Lee, Hak-Kyo; Cho, Byung-Wook; Kim, Heui-Soo

Abstract

Quantitative analysis of horse gene expression profiles under diverse experimental conditions is limited by the lack of reliable reference genes for normalization of mRNA levels. Therefore, in this study, the expression of potential reference genes was compared between thoroughbred and Jeju native horse (Jeju pony). We compared the expression of nine genes by quantitative real-time RT-PCR in fourteen tissues between the two horse breeds and analyzed their stability using the geNorm and NormFinder programs. The data obtained in this study suggest that the UBB gene could serve as a reference gene in gene expression analysis of thoroughbred and Jeju native horses.