Anti-Ubiquitin monoclonal antibody [Biotin] (DCABY-4303)

Mouse Anti-Human Ubiquitin monoclonal antibody for WB, ELISA(Det)

Specifications


Host Species
Mouse
Antibody Isotype
IgG2b
Clone
94537
Species Reactivity
Human
Immunogen
E. coli-derived recombinant human Ubiquitin+1
Conjugate
Biotin

Applications


Application Notes
Western Blot: 1 μg/mL; ELISA Capture: 2-8 μg/mL; ELISA Detection: 0.5-2.0 μg/mL
We recommend the following for sandwich ELISA (Capture - Detection):
DCABY-4229 - DCABY-4303
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
UBB; ubiquitin B; polyubiquitin-B; polyubiquitin B
Entrez Gene ID
UniProt ID

Product Background


Pathway
APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Cyclin B; APC/C:Cdc20 mediated degradation of Securin; APC/C:Cdc20 mediated degradation of mitotic proteins; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cd

Citations


Have you cited DCABY-4303 in a publication? Let us know and earn a reward for your research.

Related Products


Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

References


EDUCATIONAL MARKETING - ACADEMIC ACTION AND IDENTITY

ERD 2017 - EDUCATION, REFLECTION, DEVELOPMENT, FIFTH EDITION

Authors: Precup, Angela; Chis, Olga

The marketing practice proves that the biggest actors on the market, despite their leading position, develop consistent and sustainable communicational campaigns, in order to consolidate and increase their status. It is the case of Babes - Bolayi University (UBB) in Cluj-Napoca - the oldest, the biggest and the most prestigious university in Romania from several points of view. On this basis, the present study analyses the particular case of the university's Extension in Targu-Mures, belonging to the UBB Faculty of Psychology and Educational Sciences, in terms of brand awareness, compared to the tutelary organisation. From the conceptual perspective, the analysis reveals significant awareness differences between the two entities, in favour of the tutelary institution, although the Extension is identified with its reputation, practice and organisational culture. On the action level, the study presents an educational marketing plan, targeting to improve the Extension's awareness and connection to the local community, as well as some of its earliest results. The project scope, derived from the initial needs analysis, was to consolidate the presence of UBB - Mures Extension in its local space of reference and to increase its awareness, on a medium-term basis, by means of organizational communication and educational marketing, in order to support the organization's credibility and attractiveness among the local providers of educational services. (c) 2018 Published by Future Academy www.FutureAcademy.org.UK

EFFECT OF AMINO-ACID LIMITATION ON THE EXPRESSION OF 19 GENES IN RAT HEPATOMA-CELLS

FASEB JOURNAL

Authors: MARTEN, NW; BURKE, EJ; HAYDEN, JM; STRAUS, DS

We showed previously that the abundance of serum albumin mRNA is decreased in H4-II-E rat hepatoma cells limited for a single essential amino acid (phenylalanine, methionine, leucine, or tryptophan). To define the specificity of this phenomenon, we examined the effect of amino acid limitation on the abundance of mRNAs for 19 genes in the H4-II-E cells. These genes included six genes whose expression is either completely liver-specific or highly enriched in the liver compared with other tissues [albumin, transthyretin (TTR), transferrin, carbamyl phosphate synthetase-I, urate oxidase, class I alcohol dehydrogenase], as well as a number of ubiquitously expressed ''housekeeping'' genes. The results indicated that the 19 genes could be divided into three classes based on their response to amino acid limitation. Class I genes (the six liver-specific genes and alpha-tubulin) exhibit decreased expression in response to amino acid limitation. The expression of class II genes [beta(2)- microglobulin, hypoxanthine-guanine phosphoribosyl transferase (HPRT), H-ferritin, ubiquitin (UbB), insulin-like growth factor binding protein-4, HNF-1 alpha] is not significantly affected by amino acid limitation. Class III genes [gadd153, beta-actin, ubiquitin (UbC), phosphoglycerate kinase-1, C/EBP alpha, C/EBP beta] exhibit increased expression in response to amino acid limitation. Thus, specific inductive as well as repressive effects on gene expression are quite common in amino acid-limited cells. The observation that all six genes whose expression is liver-specific exhibited decreased expression in amino acid-limited cells suggests a common mode of regulation of these genes by amino acid availability. The strong induction by amino acid limitation of the C/EBP inhibitor gadd153 is of interest in this regard, as increased levels of gadd153 could interfere with C/EBP, which is required for high expression of most liver-specific genes. To investigate further the molecular mechanism for the decrease in albumin mRNA abundance, albumin nuclear transcript levels were quantified in control and tryptophan-limited cells. Tryptophan limitation caused a decrease in albumin nuclear transcript abundance, and this decrease preceded the decrease in albumin mRNA, suggesting that the decrease in albumin mRNA was caused at least partly by a decrease in albumin gene transcription. Additional experiments with actinomycin D indicated that albumin mRNA was also destabilized in the tryptophan-limited cells. Thus, the overall results indicate that the decrease in albumin mRNA in the tryptophan-limited cells is caused by a specific decrease in albumin nuclear transcript abundance and destabilization of albumin mRNA.

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:

Related Products

Related Resources

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

Inquiry Basket