Anti-TNFRSF1B monoclonal antibody (DCABH-201)

Rabbit Anti-TNFRSF1B monoclonal antibody for WB, ICC/IF, IHC, FC, IP Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
KN224-12
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:500-1:2,000; ICC: 1:50-1:200 IHC: 1:50-1:200; FC: 1:50-1:100; IP: 1:10-1:50
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of TNF Receptor II on different cells lysates using anti-TNF Receptor II antibody at 1/500 dilution. Positive control: Line 1: MCF-7; Line 2: Jurkat
Fig2: ICC staining TNF Receptor II in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining TNF Receptor II in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining TNF Receptor II in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue anti-TNF Receptor II antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of HL-60 cells with TNF Receptor II antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

Target


Alternative Names
CD120b; p75; p75 TNF receptor; p75TNFR; p80 TNF alpha receptor; p80 TNF-alpha receptor

Citations


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References


Zhu, XS; Bagchi, A; et al. Toll-like receptor 2 activation by bacterial peptidoglycan-associated lipoprotein activates cardiomyocyte inflammation and contractile dysfunction. CRITICAL CARE MEDICINE 35:886-892(2007).
Ali, S; Starwalt, R; et al. Tumor Necrosis Factor Is Critical for Cytolytic T Cell Activity Against Allospecific Hepatocytes and Splenic Targets in Major Histocompatibility Complex Class I Disparate Graft Versus Host Disease. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH 31:423-431(2011).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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