Anti-NCAM1 monoclonal antibody (CAB-5920RH)
Rabbit Anti-NCAM1 monoclonal antibody for WB, ICC/IF, IHC, IP, FC
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Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: Western blot analysis of NCAM on different lysates using anti-NCAM antibody at 1/1,000 dilution.
Lane 1: SH-SY-5Y;
Lane 2: Human brain
Fig2: ICC staining NCAM in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining NCAM in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCAM antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded zebrafish kidney tissue using anti-NCAM antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of SH-SY-5Y cells with NCAM antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody
antigen MSK39 identified by monoclonal antibody 5.1H11; antigen recognized by monoclonal antibody 5.1H11; CD56; cell adhesion molecule, neural, 1; MSK 39; MSK39
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Korbling, M; et al. Collection of allogeneic peripheral blood stem cells. BEST PRACTICE & RESEARCH CLINICAL HAEMATOLOGY 12:41-55(1999).
Tian, L; Catt, JW; et al. Expression of immunoglobulin superfamily cell adhesion molecules on murine embryonic stem cells. BIOLOGY OF REPRODUCTION 57:561-568(1997).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.