Human Neural cell adhesion molecule 1(NCAM1) ELISA Kit

Name: Human Neural cell adhesion molecule 1(NCAM1) ELISA Kit
SKU: DEIA-NS2625
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, cell culture supernates, cell lysates

Species Reactivity

Human

Detection Method

Sandwich-ELISA

Intended Use

For the quantitative determination of human neural cell adhesion molecule 1 (NCAM1) concentrations in serum, plasma, cell culture supernates, tissue homogenates.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Assay plate	96 wells	2-8°C
2	Standard (Freezedried)	2 vials	2-8°C/-20°C
3	Biotin-antibody (100× concentrate)	1×120μl	2-8°C/-20°C
4	HRP-avidin (100× concentrate)	1×120μl	2-8°C/-20°C
5	Biotin-antibody Diluent	1×15ml	2-8°C
6	HRP-avidin Diluent	1×15ml	2-8°C
7	Sample Diluent	1×50ml	2-8°C
8	Wash Buffer (25× concentrate)	1×20ml	2-8°C
9	TMB Substrate	1×10ml	2-8°C
10	Stop Solution	1×10ml	2-8°C
11	Adhesive Strip	4

Storage

Unopened kit: Store at 2-8°C. Do not use the kit beyond the expiration date.
Opened kit: Refer to the "Reagents and Materials Provided" section for storage instructions.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Detection Range

31.25-2000 pg/ml

Sensitivity

The minimum detectable dose of human NCAM1 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Citations