Anti-LEP polyclonal antibody

In the construction of the Loess Plateau in China, high-filled cut-and-cover tunnels (HFCCTs) had solved the problem of the shortage of land resources. However, this type of structure has a large amount of backfill soil, which leads to the problems of ultrahigh earth pressure and safety of the cut-and-cover tunnels (CCTs) lining structure. Previous studies have focused on the load reduction of various flexible materials, ignoring the influence produced by the shape of the CCT structure on the load reduction. Therefore, via a discrete element software, we investigated the changes of vertical earth pressure (VEP), vertical displacement, lateral earth pressure (LEP), and load transfer mechanisms around a HFCCT with consideration to two cases: (1) different shape of CCT structure; (2) the coupling of load reduction using expanded polystyrene (EPS) and the modified shape of the CCT lining structure. The results obtained by the discrete element method (DEM) revealed that an appropriate structural shape influenced the reduction of the VEP above the CCT and that the coupled effects of the load reduction using the EPS and shape modifications of the CCT lining structure could significantly reduce the VEP above the CCT, which enhanced the safety of the CCT. Meanwhile, the optimal values for the shapes of CCTs are derived.

The study was conducted with the objective to evaluate the effect of bamboo vinegar powder (BV) on growth performance and expression of growth-related genes in finishing pigs. Ninety pigs (140-d-old, 80.88 +/- 0.82 kg) were randomly allocated to five groups and were fed on a basal diet alone (CON; served as a control), or supplemented with either antibiotics (AB) or BV at 0.5 (BV-0.5), 1.0 (BV-1.0) and 1.5 (BV-1.5) per cent levels. Relative growth parameters and nutrient apparent digestibility were measured. Expression of IGF-1, GHR, and LEP in muscle, liver, and adipose tissues were quantified by RT-q PCR. Addition of 1.0% BV significantly (P<0.05) increased ADG and reduced FCR of finishing pigs than CON. Additionally, the CP digestibility in BV-1.0 and BV-0.5 notably improved (P<0.05) than those in other groups. The pigs showed significantly (P<0.05) elevated levels of IGF-1 and GHR mRNA expression in liver in BV-1.0 and BV-1.5 than that in other groups. The IGF-1 also showed significant higher (P <0.05) expression in adipose tissues in BV-1.0 and BV-1.5 when compared to other groups. The expression of LEP increased significantly (P<0.05) in muscle and liver tissues in BV-1.0 than that in other groups. Overall, it is concluded that BV can be supplemented at 1.0% level to promote growth and development in finishing pigs.