Anti-Human CD8A monoclonal antibody (DMAB5560RH)

Rabbit Anti-Human CD8A monoclonal antibody for WB, ICC/IF, IHC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TJ29-12
Species Reactivity
Human
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of CD8 alpha on different lysates using anti-CD8 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: Hela; Lane 2: Lovo
Fig2: ICC staining CD8 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD8 alpha in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. Counter stained with hematoxylin.

Target


Alternative Names
alpha polypeptide (p32); CD8; CD8 antigen alpha polypeptide; CD8 antigen alpha polypeptide (p32); CD8a; CD8A antigen
Entrez Gene ID
UniProt ID

Citations


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References


Nakatsuji, T; et al. A homozygous and a heterozygous defect of the winged helix DNA-binding domain of the fork head (FH) gene in tailless and tailed rat siblings: resultant lymphocyte-rich thymoma connected with renal tubule amyloid beta (A beta) deposits. CLINICAL BIOCHEMISTRY 36:171-176(2003).
Badie, B; Schartner, JM; et al. Dexamethasone-induced abolition of the inflammatory response in an experimental glioma model: a flow cytometry study. JOURNAL OF NEUROSURGERY 93:634-639(2000).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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