Anti-Human CD8A monoclonal antibody (DMAB5560RH)
Rabbit Anti-Human CD8A monoclonal antibody for WB, ICC/IF, IHC
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Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: Western blot analysis of CD8 alpha on different lysates using anti-CD8 alpha antibody at 1/1,000 dilution.
Lane 1: Hela;
Lane 2: Lovo
Fig2: ICC staining CD8 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD8 alpha in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. Counter stained with hematoxylin.
alpha polypeptide (p32); CD8; CD8 antigen alpha polypeptide; CD8 antigen alpha polypeptide (p32); CD8a; CD8A antigen
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Juul-Madsen, HR; Jensen, KH; et al. Ontogeny and characterization of blood leukocyte subsets and serum proteins in piglets before and after weaning. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 133:95-108(2010).
Kouchaki, E; Salehi, M; et al. Numerical status of CD4(+)CD25(+)FoxP3(+) and CD8(+)CD28(-) regulatory T cells in multiple sclerosis. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 17:250-255(2014).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.