Animals were immunized with mouse splenic or thymic lymphocytes. Spleen cells were fused with NS-1 myeloma cells. Tested and found negative for ectromelia virus (mousepox).
Application Notes
Can be used in a sandwich killing cytotoxicity assay when conjugated with arsanilic acid and used in conjunction with rabbit anti arsanilic acid antibody.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2-3 x 10^5 cells/mL and maintain cultures at or below 1 x 10^6 cells/ml.
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