Animals were immunized with the BM10-5.6 cloned CTL (cytotoxic T lymphocyte) line. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with Lyt 2.2. The antibody will fix complement.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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