Anti-Human CD3e monoclonal antibody (DMAB5553RH)
Rabbit Anti-Human CD3e monoclonal antibody for FC, IHC-P, WB
Datasheet
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Specifications
Species Reactivity
Human, Rat
Immunogen
synthetic peptide KAKAKPVTRGAGA, corresponding to amino acids 156-168 of Human CD3 epsilon chain.
Applications
Application Notes
Recommended dilution:
FC: Assay-Dependent
IHC-P: Assay-Dependent
WB: Assay-Dependent
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Data Examples
Immunohistochemical analysis of CD3e in formalin-fixed, paraffin-embedded human tonsil tissue using a CD3E monoclonal antibody. Detection was performed with a peroxidase-conjugate and AEC chromagen. Note cell membrane staining of T cells.
Target
Alternative Names
CD3, CD3 epsilon
Citations
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References
Li, Q; Minami, M; et al. Acute immunotoxicity of p-chloronitrobenzene in mice: II. Effect of p-chloronitrobenzene on the immunophenotype of murine splenocytes determined by flow cytometry. TOXICOLOGY 137:35-45(1999).
Feng, RQ; Tanaka, H; et al. Human T-cell leukemia virus type 1 can infect a wide variety of cells in mice. JAPANESE JOURNAL OF CANCER RESEARCH 90:48-54(1999).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.
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