General Notes
This product (Catalog No. DPABH-20077) is a rabbit-derived polyclonal antibody raised against human HMGCL, targeting the amino acid region 1–325 of the protein. This antibody exhibits cross-reactivity with Human HMGCL from mouse and rat. It has been affinity purified by Protein A. HMGCL (3-Hydroxy-3-Methylglutaryl-CoA Lyase) is a mitochondrial enzyme that plays a key role in ketogenesis and leucine catabolism. It catalyzes the final step in ketone body synthesis by converting HMG-CoA into acetoacetate and acetyl-CoA, which are critical energy sources during fasting, prolonged exercise, or carbohydrate restriction. This reaction is essential for maintaining energy homeostasis, particularly in tissues such as the liver, where ketone bodies are produced and subsequently utilized by peripheral organs like the brain and muscle. HMGCL is also involved in the breakdown of the amino acid leucine, linking amino acid metabolism to energy production. Loss-of-function mutations in HMGCL cause HMG-CoA lyase deficiency, a rare metabolic disorder characterized by hypoketotic hypoglycemia, metabolic acidosis, and impaired ketone body formation. Overall, HMGCL is a vital metabolic enzyme for energy balance and ketone body biosynthesis.
DPABH-20077 is suitable for use in Western blot (WB) (1:1,000–1:6,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:20–1:200) applications.
DPABH-20077 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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