Anti-Gastrin 1 (C-terminal) polyclonal antibody

This paper discusses reciprocal constructions in Hittite, framed within the typology of reciprocals laid out by Nedjalkov (2007), Konig & Gast (2008), and Evans et al. (2011). Hittite attests to at least three reciprocal markers, that is, the middle voice, the particle = za, and three different polyptotic constructions, based on the iteration of the numeral sia- 'one', the demonstrative ka- 'this', and the noun ara- 'fellow, comrade' respectively. Synchronically, these three strategies cover different sub-domains of reciprocity, as they encode different kinds of reciprocal situations and display different syntactic properties. These differences can be accounted for by taking into account the processes out of which these constructions developed, which can be described in terms of grammaticalization (Heine & Miyashita 2008). In this respect, beside enriching our knowledge of reciprocals in Indo-European languages, this paper also contributes to our general understanding of the diachronic typology of reciprocal constructions.

The central variable region (CVR) of the African swine fever virus (ASFV) genome is contained within the 9-RL open reading frame (ORF). ORF 9-RL of the ASFV isolate Malawi Lil-20/1 predicts a protein of 614 amino acids with amino- and carboxy-terminal hydrophobic regions and a centrally located hydrophilic region. The CVR of the genome, located centrally within this ORF, is 372 bp and contains a 132-bp direct repeat. The translated CVR within ORF 9-RL contains 31 tandem tetramers, predominantly NADT, NANT, NVDT, and, in a few cases, CAST, GAST, or CADT. In vitro translation of 9-RL yielded a 94-kDa protein that was strongly reactive with convalescent pig serum while monospecific 9-RL antiserum identified a late viral protein of 94 kDa in ASFV-infected macrophages. The protein, detected by immunofluorescence staining with 9-RL antiserum, was distributed homogeneously throughout the cytoplasm of infected Vero cells. 9-RL protein size varied among different viral isolates and among cell-culture-adapted viruses. Protein size increased proportionately with the degree of cell culture adaptation and was directly correlated with the size of the CVR present within the ORF (300 - 500 bp). Analysis of the number and composition of tandem tetramers present within the CVR of a given ASFV isolate may prove useful for identifying and/or grouping ASFV isolates. (C) 1996 Academic Press, Inc.