Gastrin ELISA Kit (DEIA-XYA969)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cultured cells
Species Reactivity
Human, Mouse, Rat
Intended Use
The Gastrin Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Gastrin protein expression profile in cells. The kit can be used for measuring the relative amounts of Gastrin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Gastrin.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-Gastrin Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months

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References


Business Aircraft Multipath Analysis for GBAS CAT II/III

PROCEEDINGS OF THE 2014 INTERNATIONAL TECHNICAL MEETING OF THE INSTITUTE OF NAVIGATION

Authors: Dvorska, J.; Musil, M.; Orejas, M.; Kutik, O.

The paper focuses on multipath analyses for business aircraft as the current GBAS RTCA multipath model is based on data collected for mainline aircraft. The key objective is to characterize airframe multipath errors and validate that they meet the RTCA model requirement for a typical business aircraft installations, using real data collected during flight as well as simulations to extend the results for more aircraft. In order to collect data for business aircraft multipath analysis, Honeywell flight test aircraft King Air 200 was equipped with three GNSS receivers. The recording equipment included two aviation grade receivers (one with 0.2 chips correlator spacing, the other with adjustable 0.1 or 0.2 chips) and one dual-frequency receiver as a reference. The flight plan covered typical manoeuvres that are encountered in normal operations. There is a particular need for repeated approaches in order to evaluate the multipath for regions where GAST D is applicable. In first campaign, there were a total of 30 flights with all three receivers connected to one dual-frequency antenna. Of those, 15 were flown with the 0.1 chip correlator and 15 with the 0.2 chip correlator for the receiver with adjustable spacing. In the second part, there were a total of 19 flights with the avionics receivers connected to a different single-frequency antenna, then the truthing system. On average, each flight lasted about two hours. The data were analyzed separately in different phases (ground, air, on approach) because multipath effects can be very different when the aircraft is on the ground or airborne. The approach phase which is a subset of the air phase was extracted and analyzed separately. In order to estimate the multipath error, it is necessary to eliminate all the other contributions of the total error. Since multipath is one of the smaller contributions, its isolation requires accurate cancelation of other effects. One of the commonly used techniques is the code-minus-carrier (CMC) in which the common error terms cancel out leaving only the ionospheric divergence, carrier ambiguity, multipath and thermal noise effects. The divergence-free CMC is more suitable for the estimation of code noise and multipath in the L1 measurement because it does not contain the L2 pseudorange error which is not of interest in this analysis. In order to eliminate thermal noise, GNSS simulator was used, to measure CMC versus carrier-to-noise. The multipath plus noise values are compared to the multipath + AAD B model (Airborne Accucy Designator B; minimum and maximum signal levels), and the estimated multipath values are compared to the multipath model. On the ground (during taxi and while stationary), the pseudorange error is the highest because of reflections from the ground and airport structures. The air phase exhibits values which are about half of those seen on the ground. However, they are slightly above the RTCA model in some elevation bins. For the approach phase, the pseudorange error is the lowest and just below the GAST D requirement across all elevations. A legacy multipath diffraction model was tailored for business aircraft. The diffraction model is generated based on shape and size of actual aircraft. Its performance was modified to fit the results of collected data. The analyses have shown that for small aircraft, such as King Air, there is no benefit for multipath mitigation in the use of 0.1 correlator instead of 0.2 correlator in the airborne receiver. The measured results show the performance to be just below the requirement on the approach, i.e. the multipath model currently in the SARPS is applicable as well to a small business aircraft and not only mainline aircraft for which it was originally tailored. This can be extended to other business aircraft. Preliminary analysis using diffractor model has been performed on larger business aircraft that will be used during validation flight tests and indicate results that are conforming with the current requirements.

Specialty fiber gratings for sensing GAST (Gamma dose, Axial Strain and Temperature)

2012 INTERNATIONAL CONFERENCE ON FIBER OPTICS AND PHOTONICS (PHOTONICS)

Authors: Kher, Sanjay; Chaubey, Smita; Kishore, Jai; Oak, S. M.

Multi-parameter sensing specialty fiber gratings are of crucial importance for nuclear industry due to their unique properties such as high radiation dose sensitivity, viability of operation up-to 1 MGy dose. We report on our unique in-situ dose measurements of Turnaround-point LPGs and their applications for strain and temperature measurements.

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