Anti-GH1 monoclonal antibody, clone 9H7

Linker histones (H1s) are conserved and ubiquitous structural components of eukaryotic chromatin. Multiple non-allelic variants of H1, which differ in their DNA/nucleosome binding properties, co-exist in animal and plant cells and have been implicated in the control of genetic programs during development and differentiation. Studies in mammals and Drosophila have revealed diverse post-translational modifications of H1s, most of which are of unknown function. So far, it is not known how this pattern compares with that of H1s from other major lineages of multicellular Eukaryotes. Here, we show that the two main H1 variants of a model flowering plant Arabidopsis thaliana are subject to a rich and diverse array of post-translational modifications. The distribution of these modifications in the H1 molecule, especially in its globular domain (GH1), resembles that occurring in mammalian H1s, suggesting that their functional significance is likely to be conserved. While the majority of modifications detected in Arabidopsis H1s, including phosphorylation, acetylation, mono and dimethylation, formylation, crotonylation and propionylation, have also been reported in H1s of other species, some others have not been previously identified in histones.

A Gram-negative, motile, aerobic and rod-shaped bacterial strain designated 119BY6-57(T) was isolated from spongin. The taxonomic position of the novel isolate was confirmed using the polyphasic approach. Strain 119BY6-57(T) grew well at 25-30A degrees C on marine agar. On the basis of 16S rRNA gene sequence similarity, strain 119BY6-57(T) belongs to the family Xanthomonadaceae and is related to Lysobacter aestuarii S2-C-T (99.8% sequence similarity), L. maris KMU-14(T) (97.5%), and L. daejeonensis GH1-9(T) (97.3%). Lower sequence similarities (97.0%) were found with all of the other recognized members of the genus Lysobacter. The G + C content of the genomic DNA was 69.9 mol%. The major respiratory quinone was Q-8 and the major fatty acids were C-16:0 iso, C-15:0 iso, summed feature 9 (comprising C-17:1 iso omega 9c and/or C-16:0 10-methyl), summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c), and C-11:0 iso 3-OH. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three unidentified phospholipids, and an unidentified polar lipid. DNADNA relatedness values between strain 119BY6-57(T) and its closest phylogenetically neighbors were below 48.0 +/- 2.1%. Based on genotypic and phenotypic characteristics, it is concluded that strain 119BY6-57(T) is a new member within the genus Lysobacter, for which the name Lysobacter spongiae sp. nov. is proposed. The type strain is 119BY6-57(T) (= KACC 19276(T) = LMG 30077(T)).