Mouse/Rat Growth Hormone ELISA Kit

Sample

Serum and plasma

Species Reactivity

Mouse and rat

Detection Method

sELISA

Intended Use

Mouse/Rat Growth Hormone ELISA Kit is intended to be used for the measurement of Growth Hormone in mouse and rat serum and plasma samples for research use.

Contents of Kit

1. Microtiter plate: ready for use, coated with goat-anti-mouse/rat-GH-antibody. Wells are separately breakable. (8x12) wells
2. Standards (A-G): lyophilized, (recombinant rat GH), concentrations are given on vial labels and on quality certificate. 7 x 1 mL
3. Control Serum 1 (KS1): lyophilized, (rat serum), concentration is given on quality certificate. 1 x 150 μL
4. Control Serum 2 (KS2): lyophilized, (rat serum), concentration is given on quality certificate. 1 x 150 μL
5. Antibody Conjugate (AK): ready for use, guinea pig anti-m/r-GH-Antibody, biotinylated. 1 x 12 mL
6. Enzyme Conjugate (EK): ready for use, contains HRP (Horseradish-Peroxidase)-labeled Streptavidin. 1 x 12 mL
7. Dilution Buffer (VP): ready for use. Please shake before use! 1 x 50 mL
8. Washing Buffer (WP): 20-fold concentrated solution. 1 x 50 mL
9. Substrate (S): ready for use, horseradish-peroxidase-(HRP) substrate, stabilised Tetramethylbencidine. 1 x 12 mL
10. Stopping Solution (SL): ready for use, 0.2 M sulfuric acid. 1 x 12 mL
11. Sealing Tape: for covering the microtiter plate. 3

Storage

Store the kit at 2-8°C after receipt until its expiry date. The lyophilized reagents should be stored at –20 °C after reconstitution. Avoid repeated thawing and freezing.
The shelf life of the components after initial opening is warranted for 4 weeks, store the unused strips and microtiter wells airtight together with the desiccant at 2-8°C in the clip-lock bag, use in the frame provided. The reconstituted components standards A-G and Control Sera KS1 and KS2 must be stored at –20°C (max. 4 weeks). For further use, thaw quickly but gently (avoid temperature increase above room temperature and avoid excessive vortexing). Up to 3 of the freeze-thaw cycles did not influence the assay. The 1:20 diluted Washing Buffer WB is 4 weeks stable at 2-8°C

Precision

The Inter- and Intra-Assay variation coefficients were on average <10% and <5%.
Inter-Assay-Variation (n=7)

Intra-Assay-Variation (n=12)

Sensitivity

The analytical sensitivity of the Mouse/Rat Growth Hormone ELISA Kit was measured by the variability of the signal of the blank (by 15 to 16-fold determinations). Based on the twofold standard deviation of the blank the mean analytical sensitivity is < 0.04 ng/mL (Range 0.014 to 0.054 ng/mL).

Standard Curve

The exemplary shown standard curve in Figure below cannot be used for calculation of your test results. You have to establish a standard curve for each test you conduct!