Growth Hormone (rat) EIA Kit

Bacterial blight disease caused by Psendomonas syringae pv. glycinea becomes one among several important factors that affects soybean production. To control this pathogen, most farmers apply synthetic pesticides that have negative effect for human, animal, and environment. Therefore, an alternative control must be developed including the use of bacteriophages. Moreover, identification and detection of this pathogen should be accurate for optimal diseases management. On the other hand, bacteriophage, a virus that infects specific host-bacterium, is largely known to have the ability to kill their specific hostbacterium. In addition, bacteriophage is also used as diagnostic tool for bacterial detection and identification of purpose, known as phatovar classification. This research was aimed to isolate the bacterial blight pathogen and their bacteriophage from soybean field in Jember, determine the pathogen pathogenicity and virulence, and determine the host-range of bacteriophage that infects several isolates of bacterial blight pathogen on soybean. The results has shown that among 12 isolates of bacterial blight pathogens that had similar characteristic to P. syringae pv. glycinea, only 10 isolates showed positive hypersensitive response (HR) on tobacco leaf. However, all isolates were virulent when inoculated to soybean leaves by showing particular bacterial blight symptom. In addition, one bacteriophage was found to infect isolate H3, called phi PSGH3, and was to have 6 hosts among 12 bacterial isolates including isolates H3, SK4-2, BT4-1, KR1-1, KR4-2 dan MG4-1. The results from plaque assay PSGH3 with phi H3-1 found out three only particle bacteriophage which are phi GH1, phi GH2 and phi GH3. (C) 2014 The Authors. Published by Elsevier B.V.

The classical route for second-generation ethanol from lignocellulosic biomass is hampered by high process costs, fostering the development of alternative strategies such as simultaneous saccharification and fermentation (SSF). However, the lack of compatible enzyme cocktails poses a challenge. In this study, the enzymeEmBgl from the marine bacteriumExiguobacterium marinumwas rationally identified based on structural and phylogenetic analyses, known desirable properties of close orthologs, and the ecological niche of its organism source.EmBgl is a multifunctional and glucose-tolerant enzyme that efficiently hydrolyzes cello-oligosaccharides due to a positive-subsite region that can accommodate long cello-oligosaccharides without imposing steric impediments. The efficacy ofEmBgl in an SSF process was demonstrated using pretreated sugarcane bagasse as feedstock, yielding 28 g L(-1)of ethanol in 30 h. The distinguishing functional properties ofEmBgl and its successful utilization in an SSF process highlight its potential in biotechnological applications in which lignocellulose deconstruction is desirable under milder temperatures (c) 2020 Society of Chemical Industry and John Wiley & Sons, Ltd.