Anti-eIF4A3 monoclonal antibody, clone FQS25412(C)

Antibody Isotype

IgG

Clone

FQS25412(C)

Species Reactivity

Mouse; Rat; Human

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF4A3 aa 50-150. The exact sequence is proprietary.Database link: P38919

Conjugate

Application Notes

WB: 1/1000 - 1/2000; Flow Cyt: 1/160; IHC-P: 1/100; ICC/IF: 1/500; IP: 1/30;

Alternative Names

EIF4A3; eukaryotic translation initiation factor 4A3; DDX48, DEAD (Asp Glu Ala Asp) box polypeptide 48 , eukaryotic translation initiation factor 4A, isoform 3; eukaryotic initiation factor 4A-III; EIF4AIII; KIAA0111; NMP 265; hNMP 265; eIF4A-III; eIF-4

Entrez Gene ID

UniProt ID

Antigen Description

ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.

Pathway

Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Deadenylation of mRNA, organism-specific biosystem; Deadenylation-dependent mRNA decay, organism-specific biosystem; Exon junction complex (EJC), organism-specific biosystem; Gene Expression, organism-specific biosystem; ISG15 antiviral mechanism, organism-specific biosystem;