Cytomegalovirus proteins, maternal pregnancy cytokines, and their impact on neonatal immune cytokine profiles and acute lymphoblastic leukemogenesis in children
Joseph L Wiemels, Rong Wang, Mi Zhou, Helen Hansen, Rachel Gallant, Junghyun Jung, Nicholas Mancuso, Adam J De Smith, Catherine Metayer, Scott C Kogan, Xiaomei Ma
Applications: IA
Reactive species: Cytomegalovirus
"Abstract: Early cytomegalovirus (CMV) infection and altered cytokine profiles at birth are associated with risk of childhood acute lymphoblastic leukemia (ALL).1-5 We examined neonatal cytokine levels and CMV proteins in 130 children who contracted ALL later in life and 460 controls. We assessed the immunodominant viral coat protein (pp65) and CMV proteins that manipulate human immune function (CMVIL-10, CMV-CXCL-1), which were detectable in most neonatal samples and correlated with specific cytokine levels (IL-10, IL12, TGF-β1, and TNFα) CMV-IL-10 was positively associated with ALL risk."
Article snippet: CMV protein standards (*) and blood spot extracts (10 uL/sample/test) were incubated with corresponding microspheres (5 uL/sample) at room temperature using a wash station, followed by 10 uL of 1:1,000 diluted biotinylated anti-CMV proteins antibodies (*; pp65: DPATB-H83463, Creative Diagnostics, New York, NY; *) and streptavidin-conjugated R-phycoerythrin 1:100 diluted stock (*). 
Figure 1. Pearson correlation matrix of cytokines and cytomegalovirus proteins at birth of all neonatal study subjects (n=590: 130 cases and 460 controls)
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