General Notes
This product (Catalog No. DPABH-01579) is a rabbit-derived polyclonal antibody raised against human CHEK2, targeting the amino acid region 300–540 of the protein. This antibody exhibits cross-reactivity with Human CHEK2 from mouse and rat. It has been affinity purified by Protein A. CHEK2, or checkpoint kinase 2, is a protein-coding gene located on the long arm of chromosome 22 at position 22q12.1 that encodes the CHK2 protein, a serine/threonine kinase essential for maintaining genomic integrity. The CHK2 protein functions as a key regulator in the cellular response to DNA damage, particularly double-strand breaks, where it is activated through phosphorylation by the ataxia-telangiectasia mutated (ATM) kinase. Upon activation, CHK2 phosphorylates multiple downstream targets, including the tumor suppressor p53 at serine-20 to stabilize it and induce G1/S cell cycle arrest, CDC25 phosphatases to prevent mitotic entry, and BRCA1 to facilitate homologous recombination repair, thereby promoting either DNA repair or programmed cell death (apoptosis) if damage is irreparable. This multifaceted role positions CHEK2 as a critical tumor suppressor that halts cell proliferation in the presence of genotoxic stress, ensuring fidelity in DNA replication and chromosome segregation.
DPABH-01579 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-01579 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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