Anti-BSG (aa 152-201) polyclonal antibody

Brewers' spent grain (BSG) is the major by-product of the brewing industry which remain largely unutilized despite its nutritional quality. In this study, the effects of fermentation on BSG antioxidant potential were analyzed. A biotechnological protocol including the use of xylanase followed by fermentation withLactiplantibacillus plantarum (Lactobacillus plantarum)PU1, PRO17, and H46 was used. Bioprocessed BSG exhibited enhanced antioxidant potential, characterized by high radical scavenging activity, long-term inhibition of linoleic acid oxidation and protective effect toward oxidative stress on human keratinocytes NCTC 2544. Immunolabelling and confocal laser microscopy showed that xylanase caused an extensive cell wall arabinoxylan disruption, contributing to the release of bound phenols molecules, thus available to further conversion through lactic acid bacteria metabolism. To clarify the role of fermentation on the antioxidant BSG potential, phenols were selectively extracted and characterized through HPLC-MS techniques. Novel antioxidant peptides were purified and identified in the most active bioprocessed BSG.

Basil seed gum (BSG) edible films containing with Origanum vulgare subsp. viride essential oil (OEO) (1-6%) were formulated in order to coating of fresh cut apricots. Chemical attributes, microbial load and sensory characteristics of coated fresh apricot-cut during cold storage at 4 degrees C for 8 d were investigated. The addition of OEO significantly decreased water vapor permeability (WVP) of films while increased their moisture content (p < 0.05).The applied coatings reduced total plate count, yeasts and molds populations. Among all tested treatments, the BSG +6% OEO was determined as the most effective in reducing the microbial populations of apricot cuts. In comparison with the control, total soluble phenolic and antioxidant activity of OEO added samples was enhanced significantly at the end of cold storage. The new introduced incorporated OEO BSG film and coating could provide great improvement in terms of odor and overall acceptability and it could be applied in order to maintain quality of fresh apricot cuts. (C) 2016 Elsevier B.V. All rights reserved.