Anti-BSG monoclonal antibody, clone NFN-N7/7 Functional Grade

This paper studies the production of natural red pigments by Monascus purpureus CMU001 in the submerged fermentation system using a brewery waste hydrolysate, brewer's spent grain (BSG). The chemical, structural and elemental characterization of the BSG was performed with Van-Soest method, Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), respectively. The lignocellulosic structure of BSG was hydrolyzed with a dilute sulfuric acid solution (2% (w/v)) followed by detoxification with Ca(OH)(2). Maximum red pigment production (22.25 UA(500)) was achieved with the following conditions: 350 rpm shake speed, 50 mL fermentation volume, initial pH of 6.5, inoculation ratio of 2% (v/v), and monosodium glutamate (MSG) as the most effective nitrogen source. Plackett-Burman design was used to assess the significance of the fermentation medium components, and MSG and ZnSO(4)7H(2)O were found to be the significant medium variables. This study is the first study showing the compatibility of BSG hydrolysate to red pigment production by Monascus purpureus in a submerged fermentation system.

In the presented work, rigid polyurethane-polyisocyanurate (PUR-PIR) foams filled with brewers' spent grain (BSG) were prepared. The influence of the isocyanate index (II) on its performance was investigated. Foams obtained with higher isocyanate index required a higher amount of hydrofluorocarbon physical blowing agent to provide the same apparent density of material. An increase of isocyanate index resulted in a slight decrease of cell size, which was related to the increased crosslink density due to enhanced generation of allophanate and biuret groups. Deterioration of compressive strength, from 226 to 202 kPa was observed with the rise of the isocyanate index. Dynamic mechanical analysis and swelling tests confirmed the increase of crosslink density with the increasing isocyanate index. The glass transition temperature rose from 165.7 degrees C to 193.2 degrees C. Fourier transform infrared (FTIR) analysis indicated an increase of the isocyanurate rings' content in composites with a higher isocyanate index, causing noticeable enhancement of thermal stability. The onset of degradation was shifted from 196 degrees C to 211 degrees C.