Rabbit Anti-Mouse Bcl2 (a.a. 1-236) Polyclonal antibody

Name: Rabbit Anti-Mouse Bcl2 (a.a. 1-236) Polyclonal antibody
SKU: DPABH-25089

Rabbit Anti-Mouse Bcl2 (a.a. 1-236) Polyclonal antibody for WB, IHC, IF, FC (Intra), CoIP, ELISA

Write a review

COA

SDS

Datasheet

Key features and details

Target: Mouse Bcl2
Host: Rabbit
Application: WB, IHC, IF, FC (Intra), CoIP, ELISA
Species Reactivity: Mouse, Rat

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Mouse; Rat

Immunogen

Recombinant mouse Bcl2 protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 1-236 aa of NM-009741

Conjugate

Unconjugated

Applications

Application Notes

WB: 1:500-1:3000
IHC: 1:200-1:800
FC: 0.25 μg per 10^6 cells in a 100 μl suspension

General Notes

This product (Catalog No. DPABH-25089) is a rabbit-derived polyclonal antibody raised against mouse Mouse Bcl2, targeting the amino acid region 1–236 of the protein. This antibody exhibits cross-reactivity with Mouse Bcl2 from rat. It has been affinity purified by Protein A. Bcl-2, or B-cell lymphoma 2, is a proto-oncogene that encodes a key anti-apoptotic protein central to the regulation of programmed cell death, primarily by inhibiting mitochondrial outer membrane permeabilization (MOMP) to prevent the release of cytochrome c and subsequent activation of caspases. Discovered in 1984–1985 through its association with the t(14;18) chromosomal translocation in approximately 85% of follicular lymphoma cases, Bcl-2 was the first identified regulator of apoptosis, marking a paradigm shift in understanding how inhibition of cell death contributes to oncogenesis. As the founding member of the Bcl-2 protein family, it features a globular α-helical structure with four Bcl-2 homology (BH) domains (BH1–BH4), including a hydrophobic groove that binds pro-apoptotic family members such as BAX, BAK, and BH3-only proteins to maintain mitochondrial integrity and promote cell survival under stress.
DPABH-25089 is suitable for use in Western blot (WB) (1:500–1:3,000), IHC (1:200–1:800), flow cytometry (FC) (0.25 μg per 10^6 cells in a 100 μl suspension) applications.
DPABH-25089 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

Bcl-2; B cell leukemia/lymphoma 2; Bcl 2

Entrez Gene ID

12043

UniProt ID

P10417

Citations

Have you cited DPABH-25089 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

The CC-chemokine receptor 2 is involved in the control of ovarian folliculogenesis and fertility lifespan in mice

JOURNAL OF REPRODUCTIVE IMMUNOLOGY

Authors: Amaral Santos, Alana Gessica; Ansaloni Chagas Pereira, Luiza Aparecida; Moreira Viana, Joao Henrique; Russo, Remo Castro; Almeida Campos-Junior, Paulo Henrique

Abstract

The chemokine receptor 2 (CCR2) was first described as a chemotactic factor involved in immune responses, but it also plays an essential function in several biological processes. The chemokine (C-C motif) ligand 2 (CCL2) binds to CCR2 triggering G protein-coupled receptor (GPCR) signaling in leukocytes, including activation of PI3K/Akt/mTOR, a key pathway that is also related to follicular activation and survival. However, the potential role of CCR2 in ovarian follicular physiology remain unexplored. Thus, we investigated the role of CCR2 on follicular growth during adult life and aging. Ovaries and oocytes were collected from wild type (WT) mice at 1.5 months old (mo), and CCR2 expression was observed predominantly in oocytes included in growing follicles, as well as after ovulation. Follicle populations were assessed in WT and CCR2-/- mice at 1.5 mo, and CCR2-/- mice had more primordial and less primary and secondary follicles, while there were no differences in antral follicle numbers. Pro-apoptotic genes Bax and Casp3 were downregulated, while anti-apoptotic Bcl2 was upregulated in CCR2-/- mice. To further characterize the role of CCR2 in ovarian aging, follicle populations were assessed in WT and CCR2-/- mice at 1.5, 2.5, 6, 10, and 12 mo. A larger ovarian follicular reserve at 1.5-6 mo was observed in CCR2-/- mice. Finally, CCR2-/- aged mice (6-12 mo) ovulated more oocytes than WT mice. Altogether, these data suggest that CCR2 plays an important role in the regulation of murine folliculogenesis, potentially affecting the reproductive lifespan.

Effects of karanjin on dimethylhydrazine induced colon carcinoma and aberrant crypt foci are facilitated by alteration of the p53/Bcl2/BAX pathway for apoptosis

BIOTECHNIC & HISTOCHEMISTRY

Authors: Zhang, Jingyu; Xie, Yandong; Fan, Qingling; Wang, Cheng

Abstract

We investigated the effects of karanjin on dimethylhydrazine (DMH) induced colon cancer in rats. Male Wistar rats were injected with DMH followed by dextran sodium sulfate in drinking water for 7 days. Karanjin at doses of 50,100 and 200 mg/kg was administered orally for 18 weeks. Colon tissues were investigated using TUNEL analysis of apoptosis; histopathological assessment including number of aberrant crypt foci (ACF); immunohistochemical staining for Bcl-2-associated X protein (BAX), B-cell lymphoma 2 (Bcl2), p53 and proliferating cell nuclear antigen (PCNA); and antioxidant assay in vivo. We found that treatment with karanjin inhibited formation of ACF in the colon mucosa and reduced colon lesions. Karanjin treatment also increased the antioxidants, catalase, glutathione and superoxide dismutase. Immunostaining showed that karanjin treatment reduced BAX, p53 and PCNA levels and increased Bcl2 expression. The TUNEL assay revealed that karanjin induced apoptosis in the colon mucosa. Our findings suggest that karanjin can ameliorate colon carcinogenesis in rats by regulating BAX, Bcl2 and p53 pathways.