Anti-4-Nitrophenyl AOZ polyclonal antibody

A chemiluminescence-based biochip array sensing technique has been developed and applied to the screening of honey samples for residues of banned nitrofuran antibiotics. Using a multiplex approach, metabolites of the four main nitrofuran antibiotics could be simultaneously detected. Individual antibodies specific towards the metabolites were spotted onto biochips. A competitive assay format, with chemiluminescent response, was employed. The method was validated in accordance with EU legislation (2002/657/EC, 2002), and assessed by comparison with UHPLC-MS/MS testing of 134 honey samples of worldwide origin. A similar extraction method, based on extraction of the analytes on Oasis (TM) SPE cartridges, followed by derivatisation with nitrobenzaldehyde and partition into ethyl acetate, was used for both screening and LC-MS/MS methods. The biochip array method was capable of detecting all four metabolites below the reference point for action of 1 mu g kg(-1). The detection capability was below 0.5 mu g kg(-1) for the metabolites AHD, AOZ and AMOZ; it was below 0.9 mu g kg(-1) for SEM. IC50 values ranged from 0.14 mu g kg(-1) (AMOZ) to 2.19 mu g kg(-1) (SEM). This biosensor method possesses the potential to be a fit-for-purpose screening technique in the arena of food safety technology. (C) 2011 Elsevier B.V. All rights reserved.

A liquid chromatography-mass spectrometris method was developed for the simultameous determination of the metablolites of nitrofuran antibacterial drugs such as 3-amino-2-oxaolidone (AOZ), 5-mopholino-3-amino-2-oxazolidone (AMOZ) semicabazide (SEM) and 1-aminohydantion (AHD) in animal tissues. Samples were derivatised using 2-chlorobenzaldehyde or 2-nitrobenzaldehyde, and using AMOZ-6, AOZ-d4 as internal standards cleaned up with EN SPE columns and determined by high performance liquid chromatography-mass spectrometry. The analytes were separated and analysed by gradient elution of acetonitrle and 0.1 % fomic acid in 15 minutes. The recoveries ranged from 85. 0% to 90. 0% and the detection limit was 0. 5 mu g/kg. More identified information is available by using two different derivative methods. Furthermore, the regulation of nitrofuran residues in chick feeding was studied.