Anti-4-Nitrophenyl AOZ polyclonal antibody

An AOZ method, based on high-performance liquid chromatography (HPLC), was optimized on HPLC condition such as mobile phase and wavelength to simultaneously quantify six kinds of mycotoxins [four aflatoxins (AFs), ochratoxin A (OTA) and zearalenone (ZEA)]. Conditions for immunoaffinity clean-up, HPLC and photo-derivatization were optimized in this study and successfully applied in assessment of airborne mycotoxins from a poultry house in Dalian, China. Fifty-two air samples were collected with AGI-30 air samplers using pure water as collection media. Twenty air samples (20/52, 38.46%) were positive for four toxins. Among the positive samples, airborne mycotoxin c ncentrations (mean +/- S.D.) for AFG2, AFBI, and ZEA were 0.189 +/- 0.024 (n = 9), 0.080 +/- 0.003 (n = 11) and 2.363 +/- 0.030 (n = 5) ng/m(3) air, while the concentration for OTA was 8.530 (n = 1) ng/m(3). No positive sample was found for either AFG(1) or AFB(2). A chicken may inhale 0.019-0.057ng AFG(2), 0.013-0.019ng AFB(1), 0.436-0.513ng ZEA, and 1.706ng OTA, respectively, in a day. A poultry worker may inhale 0.504-1.512 ng AFB(1), 0.752-2.28 ng AFG(2), 68.240 ng OTA, and 17.432-20.512 ng ZEA in a working day. This is the first report on airborne mycotoxins in poultry house. These data may have importance in animal and public health implications. (C) 2008 Elsevier Inc. All rights reserved.

Nitrofurans are a class of drugs, which are typically used as antibiotics or antimicrobials illegally in aquiculture. In this paper, indirect competitive enzyme-linked immunosorbent assay and strip sensor were developed for detection of furazolidone metabolite, 3-amino-2-oxazolidinone (AOZ). These two methods could detect AOZ with high sensitivity and specificity. The immunosorbent assay with the half-inhibitory concentration of 0.074ng/mL has a good recovery from 79.9% to 119.8% for detection of AOZ in pork, chicken, fish, shrimp, pork liver and chicken liver. Based on the developed strip sensor, the detection limit was 0.05ng/g by the naked eyes and the cut-off value was 0.3ng/g. The cross-reactivity with other nitrofurans, metabolites and some other compounds was all less than 0.1%. The sample recoveries ranged from 97.3% to 115.5%. Both these methods could be used for on-site detection of furazolidone metabolite at trace level in animal tissues.