Anti-Amyloid beta (phospho S8) monoclonal antibody, clone 2F5F22

Type 2 diabetes mellitus (T2DM) is a widespread chronic disease with a high prevalence of comorbidity and mortality. The exponential increase of TD2M represents an important public health challenge and leads a strong demand for the development of relevant in vitro models to improve mechanistic understanding of diabetes and identify new anti-diabetic drugs and therapies. These models involve considering the multi-organ characteristic of T2DM. The organ-on-chip technology has made it possible to connect several organs thanks to dedicated microbioreactors interconnected by microfluidic network. Here, we developed pancreas-liver coculture model in a microfluidic biochip, using rat islets of Langerhans and hepatocytes. The behavior and functionality of the model were compared to islets and hepatocytes (with/without insulin) monocultures. Compared to monoculture, the islets coculture presented high C-peptide and insulin secretions, and downregulation of Pdx1, Glut2, App, Ins1, Neurod, Neurog3 and Gcgr genes. In the hepatic compartment, the monocultures without insulin were negative to CK18 staining and displayed a weaker albumin production, compared to monoculture with insulin. The hepatocytes cocultures were highly positive to INSR, GLUT2, CK18 and CYP3A2 immunostaining and allowed to recover mRNA levels similar to monocultures with insulin. The result showed that islets could produce insulin to supplement the culture medium and recover hepatic functionality. This model illustrated the potential of organ-on-chip technology for reproducing crosstalk between liver and pancreas.

An integrated research coupling a field study with an agronomic-economic model (ANSWER-APP) was conducted to investigate the combined effects of irrigation levels and crop loads on fruit quality, water productivity and profitability of mature date palms. The study took place in Israel's hyper-arid Arava Valley, where date palm trees are widely cultivated and rely exclusively on brackish water irrigation. Three fruit load intensities (low, FL1; commercial, FL2; high, FL3) and two irrigation levels (W1 and W2, equal to and higher than the local irrigation regime, respectively) were applied in a 22-year-old date palm orchard in 2018-2019. Irrigation amount, fruit quality and yield were measured. Profitability for each treatment was analyzed with the ANSWER-APP. Higher fruit load resulted in higher yield and water productivity, but on the other hand led to reduced fruit physical properties (size and mass), regardless of the irrigation treatment. The improved fruit physical properties and net profits in W2 compared to W1 treatment in each fruit load group resulted from excess salt leaching in the root zone. Notably, trees under W2FL2 treatment were found to have highest net profits. It is concluded that adequate irrigation amounts contribute to net profits in date palm trees treated with high fruit load intensity under saline irrigation conditions.