Human sAPP-alpha high sensitive ELISA Kit

Name: Human sAPP-alpha high sensitive ELISA Kit
SKU: DEIABL363
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Plasma, Cerebrospinal Fluids and Cell Culture Supernatant

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The kit is capable for the quantitative determination human sAPPα in serum, EDTA plasma, cerebrospinal fluids and cell culture supernatant.

Contents of Kit

1. Precoated Plate
2. Labeled Antibody Conc. (30×)
3. Standard
4. ELISA Buffer
5. Solution for Labeled Antibody
6. Chromogen
7. Stop Solution
8. Wash Buffer Conc.

Storage

2 - 8°C

Performance Characteristics

1. Titer Assay (Samples with standard added are used.)

2. Added Recovery Assay

3. Intra – Assay

4. Inter – Assay

Detection Range

0.78 - 50 ng/mL

Sensitivity

0.09 ng/mL
The sensitivity for this kit was determined using the guidelines under the National Committee for Clinical Laboratory Standards (NCCLS) Evaluation Protocols.

General Description

Alzheimer's disease (AD) was first reported by A. Alzheimer, a German neuropathologist in 1907 and is considered as a major factor of dementia. It is known that Amyloid β (Aβ; which is major constituent of senile plaque) is cleaved from Amyloid Precursor Protein (APP; which exists in three main isoforms, APP695, APP751, and APP770) by β-secretase and subsequent γ-secretase. The production of soluble APPβ (sAPPβ) by β-secretase cleavage corresponds to Aβ production accordingly, so it is desired to measure sAPPβ in parallel with Aβ. In addition, it is reported that APP gene mutation exists in individuals who suffer earlyonset Alzheimer's disease. Swedish mutation, one of the APP gene mutations, is a double mutation at positions -1 to -2 from the β-secretase cleavage site (Lys670-Asn and Met671-Leu) . And further, it is reported that Swedish mutation elevates Aβ40 and Aβ42 production, and that the mutation is utilized in establishment of transgenic mice. The measuring sAPPβ in Swedish type is useful for research of AD as well as in wild type. On the one hand, it is considered that in the metabolic pathway of APP, APP is first cleaved by α-secretase rather than β-secretase normally to produce soluble APPα (sAPPα) and subsequently P3 is cleaved from the remaining C-terminal fragment by γ-secretase. This kit can measure human sAPPα in samples.

Standard Curve

*The typical standard curve is shown above. This curve can not be used to derive test results. Please run a standard curve for each assay.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

What users really think about the usability of smartphone applications: diversity based empirical investigation

MULTIMEDIA TOOLS AND APPLICATIONS

Authors: Badshah, Sher; Khan, Arif Ali; Hussain, Shahid; Khan, Bilal

Abstract

In recent years, smartphone devices are becoming progressively popular across a diverse range of users. However, user diversity creates challenges in smartphone application (app) development. The diversity of users is often ignored by designers and developers due to the absence of requirements. Owing to this, many smartphone users face usability issues. Despite that, no dedicated platform found that guide smartphone app designers and developers regarding human universality. The aim of this research is to explore the requirements of diverse users in smartphone apps and provide usability guidelines. The objectives of this research are achieved by following two scientific approaches. The human diversity requirements are located by conducting usability tests that investigated the requirements in the form of usability issues. The systematic literature review (SLR) process is followed in order to resolve the discovered usability issues. Both approaches resulted in a list of usability issues and guidelines. The usability tests returned 27 problems while the SLR came with a comprehensive set of universal usability guidelines that were grouped into eleven categories. The study concluded with some major outcomes. The results show evidence of critical usability problems that must be addressed during the design and development of smartphone apps. Moreover, the study also revealed that people with disabilities were three times severely affected by usability problems in such apps than people of different ages and their needs must be considered a top priority in the development of smartphone apps.

A Behavioral Authentication Method for Mobile Based on Browsing Behaviors

IEEE-CAA JOURNAL OF AUTOMATICA SINICA

Authors: Chen, Dongxiang; Ding, Zhijun; Yan, Chungang; Wang, Mimi

Abstract

The passwords for unlocking the mobile devices are relatively simple, easier to be stolen, which causes serious potential security problems. An important research direction of identity authentication is to establish user behavior models to authenticate users. In this paper, a mobile terminal APP browsing behavioral authentication system architecture which synthesizes multiple factors is designed. This architecture is suitable for users using the mobile terminal APP in the daily life. The architecture includes data acquisition, data processing, feature extraction, and sub model training. We can use this architecture for continuous authentication when the user uses APP at the mobile terminal.