HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The ACVR1B knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Nature
Cell Lysate
Alternative Names
ACVR1B; activin A receptor, type IB; ALK4; SKR2; ACTRIB; ACVRLK4; activin receptor type-1B; activin receptor-like kinase 4; activin A receptor, type II-like kinase 4; serine/threonine-protein kinase receptor R2;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer. After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Format
Lyophilized
Concentration
The protein concentration was determined with BCA assay.
Buffer
RIPA buffer
Preservative
None
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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