Rabbit Anti-Human VINC (Phospho-Tyr821) polyclonal antibody

Aim: The aim of this study was to analyze the effect of bovine follicular fluid on the survival, morphology and kinetic parameters of bovine thawed spermatozoa under laboratory conditions. Materials and methods: The semen from 5 bulls of proven fertility was incubated in follicular and physiological fluid for 8 hours. During this time assessment using the CASA system was performed. At the beginning and the end of incubation process evaluation by flow cytometry was conducted. Results: The results of the sperm motility assessment showed a significant decrease in the analyzed parameters both in the follicular and physiological fluid. A significant reduction in all parameters characterizing movement properties in the semen incubated in the follicular fluid was found. In the physiological fluid, a similar trend was demonstrated only for the following properties: VAP, VSL, VCL, ALH, BCF. A significant difference was found for both fluids in: VCL (p=0.026), ALH (p=0.038) and LIN (p<0.001) at the beginning of incubation. The results of the plasma membrane integrity assessment showed a statistically significant increase in the percentage of dying sperm at the 8th hour of the incubation in the follicular fluid. In the case of semen incubation in physiological fluid, a statistically significant decrease in the percentage of live non-damaged cells was found with a simultaneous increase in the subpopulation of undamaged dead cells. Conclusions: Follicular fluid rapidly accelerates the capacitation process. The results of flow cytometry support the hypothesis concerning the ability of follicular fluid to prolong sperm survival.

The aim of this study was to examine the effects of tilmicosin on rabbit spermatozoa viability and motility parameters during short-term in vitro incubation at 37 degrees C. Semen samples were collected from adult New Zealand White rabbits and diluted with eight concentrations of tilmicosin: 0.300, 0.350, 0.400, 0.466, 0.500, 0.583, 0.600 and 0.700 mg.ml(-1) (TIL 1 - 8) diluted in the physiological solution. The motility parameters were evaluated using the Computer Assisted Semen Analyzer system (Sperm Vision (R)) at five time periods: 0, 1, 2, 3 and 4 hours. Cell viability was measured using the metabolic activity MTT assay. Immediately at the beginning of incubation significantly higher values of motility (MOT) and progressive motility (PRO) were detected in samples TIL2, TIL5 and TIL7 compared to the control. Significantly lower values of tested motility parameters were observed after 1 and 3 hours of incubation. All concentrations of tilmicosin have no significantly negative effect on the parameters of the MOT, PRO and velocity curved line (VCL) after 4 hours of incubation in vitro at 37 degrees C. Concentration of tilmicosin 0.350 mg.ml(-1) has a positive impact on MOT (72.39 +/- 15.62%, p<0.001) and PRO (54.28 +/- 21.23%, p<0.01) of rabbit spermatozoa after 4 hours of incubation in vitro at 37 degrees C. Supplementation of tilmicosin led to preservation of the cell over all time periods of the in vitro incubation. The results indicate that tilmicosin could be used to semen extenders without negative effects on rabbit spermatozoa motility and viability.